抗原呈递细胞是调节性T细胞的靶标，类似于介导前房相关免疫偏离的细胞。

Antigen-presenting cells are targets of regulatory T cells similar to those that mediate anterior chamber-associated immune deviation.

作者信息

Yokoi Hidetoshi, Streilein J Wayne

机构信息

The Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA.

出版信息

Ocul Immunol Inflamm. 2004 Jun;12(2):101-14. doi: 10.1080/09273940490895317.

DOI:10.1080/09273940490895317

PMID:15512980

Abstract

PURPOSE

To identify the means by which in-vitro-generated regulatory T cells, similar to those in anterior chamber-associated immune deviation (ACAID), suppress antigen-specific T-cell responses.

METHODS

T regulators (T regs), generated by stimulating ovalbumin (OVA)-specific Tcr transgenic DO11.10 T cells with OVA-pulsed, TGF-beta2-treated peritoneal exudates cells (PEC), or their supernatants were added to OVA-pulsed PEC that were used to activate DO11.10 T cells in vitro or to suppress OVA-specific delayed hypersensitivity (DH) induction in vivo.

RESULTS

OVA-pulsed PECs exposed in vitro to TGF-beta-producing T regs or their supernatants failed to activate DO11.10 T cells in vitro, and suppressed DH in mice immunized with OVA plus adjuvant.

CONCLUSION

T cells exposed to TGF-beta2-pretreated, antigen-pulsed PECs secrete soluble factors, including active TGF-beta that regulate OVA-specific responses by forcing antigen-presenting cells to promote deviant T-cell activation in vitro and in vivo.

摘要

目的

确定体外产生的调节性T细胞（类似于前房相关免疫偏离（ACAID）中的调节性T细胞）抑制抗原特异性T细胞反应的方式。

方法

用卵清蛋白（OVA）脉冲处理、经转化生长因子-β2（TGF-β2）处理的腹腔渗出细胞（PEC）或其上清液刺激OVA特异性T细胞受体转基因DO11.10 T细胞产生的调节性T细胞（Tregs），被添加到OVA脉冲处理的PEC中，这些PEC用于在体外激活DO11.10 T细胞或在体内抑制OVA特异性迟发型超敏反应（DH）的诱导。