Villafán Horacio, Gutiérrez-Ospina Gabriel
Programa de Doctorado en Ciencias Biológicas, Unidad de Posgrado, Circuito de Posgrados, Ciudad Universitaria, Edificio D, 1erpiso, Coyoacán, Ciudad de México 04510, Mexico.
Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico.
Brain Sci. 2023 Aug 30;13(9):1261. doi: 10.3390/brainsci13091261.
The eyes provide themselves with immune tolerance. Frequent skin inflammatory diseases in young blind people suggest, nonetheless, that the eyes instruct a systemic immune tolerance that benefits the whole body. We tested this premise by using delayed skin contact hypersensitivity (DSCH) as a tool to compare the inflammatory response developed by sighted (S) and birth-enucleated (BE) mice against oxazolone or dinitrofluorobenzene at the ages of 10, 30 and 60 days of life. Adult mice enucleated (AE) at 60 days of age were also assessed when they reached 120 days of life. BE mice displayed exacerbated DSCH at 60 but not at 10 or 30 days of age. AE mice, in contrast, show no exacerbated DSCH. Skin inflammation in 60-day-old BE mice was hapten exclusive and supported by distinct CD8 lymphocytes. The number of intraepidermal T lymphocytes and migrating Langerhans cells was, however, similar between S and BE mice by the age of 60 days. Our observations support the idea that the eyes instruct systemic immune tolerance that benefits organs outside the eyes from an early age. The higher prevalence of inflammatory skin disorders reported in young people might then reflect reduced immune tolerance associated with the impaired functional morphology of the eyes.
眼睛自身具备免疫耐受性。然而，年轻盲人中频繁出现的皮肤炎症性疾病表明，眼睛会引导一种有益于全身的系统性免疫耐受。我们通过使用迟发性皮肤接触超敏反应（DSCH）作为工具，来比较有视力的（S）和出生时即摘除眼球的（BE）小鼠在10、30和60日龄时对恶唑酮或二硝基氟苯产生的炎症反应，以此来验证这一前提。60日龄时摘除眼球的成年小鼠（AE）在120日龄时也进行了评估。BE小鼠在60日龄时表现出加剧的DSCH，但在10日龄或30日龄时未出现。相比之下，AE小鼠未表现出加剧的DSCH。60日龄BE小鼠的皮肤炎症具有抗原特异性，并由不同的CD8淋巴细胞支持。然而，到60日龄时，S小鼠和BE小鼠的表皮内T淋巴细胞数量和迁移的朗格汉斯细胞数量相似。我们的观察结果支持这样一种观点，即眼睛从早期就引导系统性免疫耐受，使眼外器官受益。那么，年轻人中报告的炎症性皮肤病较高的患病率可能反映了与眼睛功能形态受损相关的免疫耐受性降低。
