León Muiños E, Pérez Del Molino M L, Lado Lado F L, Pazo Nuñez M, Pardo F
Department of Microbiology, Clinical University Hospital, Santiago de Compostela, A Coruña, Spain.
Scand J Infect Dis. 2004;36(10):724-6. doi: 10.1080/00365540410020965.
Ligase chain reaction amplification (LCx Abbott Laboratories) was used to detect the presence of M. tuberculosis in 101 adenopathy specimens obtained from 98 patients. A total of 30 cases of lymph node tuberculosis were diagnosed, and the data were compared with results obtained using conventional techniques. The sensitivity of auramine staining and culture were 50.0% and 66.7%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value of the LCx assay were 80.0, 98.6, 96.0, and 92.1% respectively. The results confirm the reliability of ligase chain reaction amplification for the detection of lymph node tuberculosis.
连接酶链反应扩增法（LCx，雅培实验室）用于检测从98例患者获取的101份腺病标本中结核分枝杆菌的存在情况。共诊断出30例淋巴结结核，并将数据与使用传统技术获得的结果进行比较。金胺染色和培养的敏感性分别为50.0%和66.7%。LCx检测的敏感性、特异性、阳性预测值和阴性预测值分别为80.0%、98.6%、96.0%和92.1%。结果证实了连接酶链反应扩增法检测淋巴结结核的可靠性。
