绿光培养的眉藻属PCC 7601（蓝细菌）藻胆体中的三种与C-藻红蛋白相关的连接多肽。
Three C-phycoerythrin-associated linker polypeptides in the phycobilisome of green-light-grown Calothrix sp. PCC 7601 (cyanobacteria).
作者信息
Glauser M, Sidler W A, Graham K W, Bryant D A, Frank G, Wehrli E, Zuber H
机构信息
Institut für Molekularbiologie und Biophysik, Eidgenössische Technische Hochschule, Zürich, Switzerland.
出版信息
FEBS Lett. 1992 Feb 3;297(1-2):19-23. doi: 10.1016/0014-5793(92)80318-b.
Microanalyses by SDS-PAGE and microsequencing demonstrate that, under green-light conditions, 3 C-phycoerythrin associated rod-linker polypeptides with different N-terminal amino acid sequences are present in phycobilisomes (PBS) from Calothrix sp. 7601 cells. Two of these polypeptides, corresponding to SDS-PAGE bands at 36 and 37 kDa, could be assigned, respectively, to the cpeC and cpcD genes found on a separate cpeCD-operon in Calothrix sp. 7601 (Federspiel, N.A. and Grossman, A.R. (1990) J. Bacteriol, 172, 4072-4081). The third C-PE rod-linker polypeptide, LR,2PE,33, requires, therefore, a third gene with the suggested locus designation 'cpeE'. A C-PE (alpha beta)6-LR,2PE,33 complex containing this third rod-linker polypeptide could be isolated from phycobilisomes and characterized. PBS from both green- and red-light cells of Calothrix contain a single, unique LRC28 rod-core linker polypeptide which is not altered during chromatic adaptation.
通过SDS-PAGE和微量测序进行的微量分析表明，在绿光条件下，来自颤藻属7601细胞的藻胆体（PBS）中存在3种与C-藻红蛋白相关的杆状连接多肽，它们具有不同的N端氨基酸序列。其中两种多肽，分别对应于SDS-PAGE上36 kDa和37 kDa的条带，可分别归属于在颤藻属7601中一个单独的cpeCD操纵子上发现的cpeC和cpcD基因（Federspiel，N.A.和Grossman，A.R.（1990）J. Bacteriol，172，4072 - 4081）。因此，第三种C-PE杆状连接多肽LR,2PE,33需要一个建议基因座命名为“cpeE”的第三个基因。含有这种第三种杆状连接多肽的C-PE（αβ）6-LR,2PE,33复合物可从藻胆体中分离并进行表征。颤藻属绿光和红光细胞的PBS都含有一种单一、独特的LRC28杆状核心连接多肽，其在色适应过程中不会改变。
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