蜱传脑炎病毒NS3蛋白的一种短形式。
A short form of the tick-borne encephalitis virus NS3 protein.
作者信息
Pugachev K V, Morozova O V, Pletnev A G
机构信息
Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk.
出版信息
FEBS Lett. 1992 Feb 3;297(1-2):67-9. doi: 10.1016/0014-5793(92)80329-f.
Using monoclonal antibodies to the tick-borne encephalitis virus (TBE) nonstructural protein NS3 two forms of this protein were revealed in TBE-infected mammalian cells: a full-length form (69 kDa) and a short form (49 kDa) which has not been observed before and was called NS3'. Recombinant plasmids were constructed and various fragments of the TBE NS3 gene were expressed in rabbit reticulocyte lysate. By analyzing immune precipitates of 35S-labeled translation products, we could monitor and localize internal cleavage of NS3, due to which the NS3' protein was generated.
利用针对蜱传脑炎病毒（TBE）非结构蛋白NS3的单克隆抗体，在TBE感染的哺乳动物细胞中发现了该蛋白的两种形式：全长形式（69 kDa）和一种以前未观察到的短形式（49 kDa），后者被称为NS3'。构建了重组质粒，并在兔网织红细胞裂解物中表达了TBE NS3基因的各种片段。通过分析35S标记的翻译产物的免疫沉淀物，我们能够监测和定位NS3的内部切割，正是由于这种切割产生了NS3'蛋白。
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