[利用反义核苷酸在体外抑制蜱传脑炎病毒RNA的翻译]
[In vitro suppression of translation of tick-borne encephalitis virus RNA using antisense nucleotides].
作者信息
Nomokonova N Iu, Gorn V V, Vlasov V V
出版信息
Mol Biol (Mosk). 1993 Mar-Apr;27(2):327-34.
PMID:8487765
Abstract
Effect of antisense oligodeoxyribonucleotides on in vitro translation of RNAs corresponding to fragments of the tick-borne encephalitis virus genome has been investigated. Sequences optimal for oligonucleotide binding and translation arrest have been identified. The most efficient oligonucleotide (17-mer) at a concentration of 2.5 microM completely arrests translation of the RNA coding for the NS3 protein.
摘要
研究了反义寡聚脱氧核糖核苷酸对蜱传脑炎病毒基因组片段对应RNA体外翻译的影响。已确定了寡核苷酸结合和翻译抑制的最佳序列。浓度为2.5微摩尔的最有效寡核苷酸（17聚体）完全抑制编码NS3蛋白的RNA的翻译。
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