Suzuki Takashi, Ota Tatsuya, Fujiyama Asao, Kasahara Masanori
Department of Biosystems Science, School of Advanced Sciences, The Graduate University for Advanced Studies (Sokendai).
Genes Genet Syst. 2004 Aug;79(4):251-3. doi: 10.1266/ggs.79.251.
The jawless fish occupy an important phylogenetic position for understanding the evolution of body plans, the origin of adaptive immunity and genome evolution in chordates. We describe here the construction of a large-insert bacterial artificial chromosome (BAC) library from the inshore hagfish, Eptatretus burgeri. The BAC library contains 93,978 clones with an average insert size of 100 kb and is estimated to represent threefold genome-equivalent coverage. The library was organized in three-dimensional pools to facilitate screening by PCR. We have screened this library by PCR and isolated several BAC clones; the average number of positive clones was compatible with the estimated genome coverage of the library. This BAC library, constructed for the first time from the jawless fish, should serve as a useful resource for the scientific community.
无颌鱼类在理解身体结构的进化、适应性免疫的起源以及脊索动物的基因组进化方面占据着重要的系统发育位置。我们在此描述了一个来自近海盲鳗（蒲氏黏盲鳗）的大插入细菌人工染色体（BAC）文库的构建。该BAC文库包含93,978个克隆，平均插入片段大小为100 kb，估计代表三倍基因组当量的覆盖率。该文库以三维池的形式构建，便于通过PCR进行筛选。我们通过PCR筛选了该文库并分离出了几个BAC克隆；阳性克隆的平均数量与文库估计的基因组覆盖率相符。这个首次从无颌鱼类构建的BAC文库应为科学界提供一个有用的资源。
