Wang Wenming, Tanurdzic Milos, Luo Meizhong, Sisneros Nicholas, Kim Hye Ran, Weng Jing-Ke, Kudrna Dave, Mueller Christopher, Arumuganathan K, Carlson John, Chapple Clint, de Pamphilis Claude, Mandoli Dina, Tomkins Jeff, Wing Rod A, Banks Jo Ann
Arizona Genomics Institute, Department of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA.
BMC Plant Biol. 2005 Jun 14;5:10. doi: 10.1186/1471-2229-5-10.
The lycophytes are an ancient lineage of vascular plants that diverged from the seed plant lineage about 400 Myr ago. Although the lycophytes occupy an important phylogenetic position for understanding the evolution of plants and their genomes, no genomic resources exist for this group of plants.
Here we describe the construction of a large-insert bacterial artificial chromosome (BAC) library from the lycophyte Selaginella moellendorffii. Based on cell flow cytometry, this species has the smallest genome size among the different lycophytes tested, including Huperzia lucidula, Diphaiastrum digita, Isoetes engelmanii and S. kraussiana. The arrayed BAC library consists of 9126 clones; the average insert size is estimated to be 122 kb. Inserts of chloroplast origin account for 2.3% of the clones. The BAC library contains an estimated ten genome-equivalents based on DNA hybridizations using five single-copy and two duplicated S. moellendorffii genes as probes.
The S. moellenforffii BAC library, the first to be constructed from a lycophyte, will be useful to the scientific community as a resource for comparative plant genomics and evolution.
石松类植物是维管植物的一个古老谱系，大约在4亿年前从种子植物谱系中分化出来。尽管石松类植物在理解植物及其基因组进化方面占据重要的系统发育位置，但该类植物尚无基因组资源。
在此，我们描述了从石松类植物江南卷柏构建大型插入细菌人工染色体（BAC）文库的过程。基于细胞流式细胞术，该物种在包括亮叶扁枝石松、数字瓶儿小草、恩氏水韭和克劳氏卷柏在内的所测试的不同石松类植物中基因组大小最小。该排列好的BAC文库由9126个克隆组成；平均插入片段大小估计为122 kb。叶绿体来源的插入片段占克隆的2.3%。基于使用5个单拷贝和2个重复的江南卷柏基因作为探针的DNA杂交，该BAC文库估计包含十个基因组当量。
江南卷柏BAC文库是首个从石松类植物构建的文库，作为比较植物基因组学和进化的资源，将对科学界有用。
