Rago Vittoria, Maggiolini Marcello, Vivacqua Adele, Palma Antonio, Carpino Amalia
Department of Cell Biology, University of Calabria, 87030 Arcavacata di Rendi, Cosenza, Italy.
Anat Rec A Discov Mol Cell Evol Biol. 2004 Dec;281(2):1234-9. doi: 10.1002/ar.a.20131.
High affinity estrogen receptors (ERs) mediate estrogen action in male reproductive tissues. The objective of the present study was the immunolocalization of estrogen receptor alpha and estrogen receptor beta in immature and mature testes of pig, a species in which the role of estrogens on gonadal function is scarcely known. Testes from 3 and 18 month-old pigs were investigated. Immunohistochemistry was performed on paraffin embedded-tissues using both mouse anti-human monoclonal IgG ERalpha and IgG ERbeta 1 isoform. Western blot analysis demonstrated antibody specificity. ERalpha staining was not observed in immature testes, but it was detected in spermatogonia, spermatocytes and in the most Leydig cells of mature testes. ERbeta immunoreactivity was observed in spermatogonia and Leydig cells of immature gonads, while it was clearly detected in spermatogonia and in spermatocytes of adult pig testes. The differential ERalpha/ERbeta expression in germ and somatic cells of the gonads suggest a role of estrogens in function and in development of pig testis.
高亲和力雌激素受体（ERs）介导雌激素在雄性生殖组织中的作用。本研究的目的是对猪的未成熟和成熟睾丸中的雌激素受体α和雌激素受体β进行免疫定位，猪是一种雌激素对性腺功能的作用鲜为人知的物种。对3个月和18个月大的猪的睾丸进行了研究。使用小鼠抗人单克隆IgG ERα和IgG ERβ 1亚型对石蜡包埋组织进行免疫组织化学分析。蛋白质印迹分析证实了抗体的特异性。在未成熟睾丸中未观察到ERα染色，但在成熟睾丸的精原细胞、精母细胞和大多数莱迪希细胞中检测到了ERα染色。在未成熟性腺的精原细胞和莱迪希细胞中观察到了ERβ免疫反应性，而在成年猪睾丸的精原细胞和精母细胞中则明显检测到了ERβ免疫反应性。性腺生殖细胞和体细胞中ERα/ERβ的差异表达表明雌激素在猪睾丸的功能和发育中起作用。
