Gist Daniel H, Bradshaw Suzanne, Morrow Carla M K, Congdon Justin D, Hess Rex A
Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221-0006, USA.
Gen Comp Endocrinol. 2007 Mar;151(1):27-33. doi: 10.1016/j.ygcen.2006.06.012. Epub 2006 Aug 28.
Portions of the reproductive tract of the male (Trachemys scripta) turtle were examined by immunocytochemistry for evidence of the capacity to produce and respond to estrogen hormones (via the expression of P450 aromatase and estrogen receptors). Aromatase was detected in both the Sertoli and Leydig cells of the testis and was expressed at different levels during the spermatogenic cycle, being highest in the quiescent testis and lowest during germ cell meiosis. ERalpha was found in the Leydig cells surrounding the seminiferous tubules as well as in the epithelial cells of the excurrent canals (rete testis, efferent ductule, and epididymis). ERbeta immunoreactivity was found in both the spermatogonia and Sertoli cells in the testis, and in the epithelial cells of excurrent canals.
通过免疫细胞化学方法对雄性红耳龟（滑龟）的部分生殖道进行检测，以寻找其产生雌激素并对雌激素作出反应能力的证据（通过细胞色素P450芳香化酶和雌激素受体的表达）。在睾丸的支持细胞和间质细胞中均检测到芳香化酶，并且在生精周期中其表达水平不同，在静止睾丸中最高，在生殖细胞减数分裂期间最低。在生精小管周围的间质细胞以及输出管道（睾丸网、输出小管和附睾）的上皮细胞中发现了雌激素受体α。在睾丸的精原细胞和支持细胞以及输出管道的上皮细胞中均发现了雌激素受体β免疫反应性。
