Bova T O, Soroka V I, Derev'ianko S V, Volkova I V, Mamchur O Ie
Mikrobiol Z. 2004 Jul-Aug;66(4):86-91.
Optimal methods of purification and concentration of teschevirus strain of the pigs of the first serotype "Chernigivsky-2372" have been chosen for development of the set of Teschen pig discase diagnosticums. It has been established that differential centrifugation with following ultracentrifugation in the gradient of saccharose density permits to deprive viruses from host cell proteins, empty virus capsides and defect virus particles. That is the method fit for purification of virus preparations for the solid-phase immunoenzyme analysis.
为开发猪捷申病诊断试剂盒，已选择了对第一血清型“切尔尼戈夫斯基 - 2372”猪捷申病毒株进行纯化和浓缩的最佳方法。已确定，先进行差速离心，随后在蔗糖密度梯度中进行超速离心，能够去除病毒中的宿主细胞蛋白、空病毒衣壳和缺陷病毒颗粒。这是一种适用于纯化用于固相免疫酶分析的病毒制剂的方法。
