Mogil'nyĭ Iu I, Arkhipov N I
Arkh Patol. 1982;44(11):69-71.
A method for making preparations for combined immunocytological examinations in light and electron microscopes is described. An infected cell culture grown on slides (a piglet kidney cell culture infected with Teschen virus) was fixed with 2.5% glutaraldehyde in phosphate buffer, pH 7.2-7.4 for 10-15 min and incubated with a conjugate (peroxidase-labeled immune serum to the causative agent of the infection under study). After postfixation, cytochemical reaction for peroxidase was performed, then the material was fixed with 2% OsO4 solution in phosphate buffer, pH 7,2-7,4, dehydrated in an ascending series of alcohols and gradually impregnated with polymerising resins (epon, araldit, etc.). The cells were embedded in foil baths, and after polymerization and separation of the slide and foil preparations with good light microscopy properties. In the light microscope, the desired parts of cells or a cell were selected, removed, cut in an ultratome and examined in electron microscope.
描述了一种用于光镜和电镜联合免疫细胞检查的标本制备方法。将生长在载玻片上的感染细胞培养物（感染捷申病毒的仔猪肾细胞培养物）用pH 7.2 - 7.4的磷酸盐缓冲液中的2.5%戊二醛固定10 - 15分钟，并用结合物（针对所研究感染病原体的过氧化物酶标记免疫血清）孵育。后固定后，进行过氧化物酶的细胞化学反应，然后将材料用pH 7.2 - 7.4的磷酸盐缓冲液中的2%四氧化锇溶液固定，在梯度乙醇中脱水，并逐渐用聚合树脂（环氧树脂、阿乐得等）浸透。细胞包埋在箔浴中，聚合后将载玻片和箔制标本分离，得到具有良好光学显微镜性能的标本。在光学显微镜下，选择细胞或细胞的所需部分，取下，在超薄切片机中切片，然后在电子显微镜下检查。
