Lu Wei, Wang Yun, Zhang Xiao-jian
Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084, China.
Huan Jing Ke Xue. 2004 Jul;25(4):167-9.
Methods of the enumeration of total bacteria and Coliform in drinking water were researched in this paper. The differences between heterotrophic plate count and direct viable count method were compared. It is concluded that the total number of bacteria in R2A medium is one order of quantity higher than the traditional plate count agar, and the results of acridine orange direct counts (AODC) is the highest. Some different staining methods in direct viable count were also compared in this paper, such as nalidixic acid, CTC staining and BacLight staining. The proportion of the live bacteria to the dead is about 10%.
本文研究了饮用水中总细菌和大肠菌群的计数方法。比较了异养平板计数法和直接活菌计数法之间的差异。得出结论:R2A培养基中的细菌总数比传统平板计数琼脂高一个数量级,吖啶橙直接计数(AODC)结果最高。本文还比较了直接活菌计数中一些不同的染色方法,如萘啶酸、CTC染色和BacLight染色。活菌与死菌的比例约为10%。