Singh Neeloo, Dube Anuradha
Division of Biochemistry and Division of Parasitology, Central Drug Research Institute, Lucknow, India.
Am J Trop Med Hyg. 2004 Oct;71(4):400-2.
Classic techniques for detecting the susceptibility of Leishmania to different drugs are time-consuming, laborious, and require the use of macrophages. The use of flow cytometry for monitoring Leishmania susceptibility to drugs is beginning to be implemented. Using green fluorescent protein (GFP), we have improved and simplified the screening procedure. We introduced a GFP marker into field strains of Leishmania causing kala-azar (visceral leishmaniasis) and explored the suitability of transgenic L. donovani promastigotes that constitutively express GFP in their cytoplasm as target cells for in vitro screening of anti-leishmanial drugs.
检测利什曼原虫对不同药物敏感性的经典技术耗时、费力，且需要使用巨噬细胞。利用流式细胞术监测利什曼原虫对药物的敏感性的方法已开始得到应用。我们利用绿色荧光蛋白（GFP）改进并简化了筛选程序。我们将GFP标记引入引起黑热病（内脏利什曼病）的利什曼原虫野外菌株中，并探索了在细胞质中组成性表达GFP的转基因杜氏利什曼原虫前鞭毛体作为抗利什曼原虫药物体外筛选靶细胞的适用性。
