Ohtake Yosuke, Maruko Akiko, Kojima Soichi, Ono Tomomi, Nagashima Tomomi, Fukumoto Manabu, Suyama Satoko, Abe Shinya, Sato Naoko, Ohkubo Yasuhito
Department of Radiopharmacy, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan.
Biol Pharm Bull. 2004 Nov;27(11):1758-62. doi: 10.1248/bpb.27.1758.
We investigated a relationship within zonal differences in DNA synthesis and in transglutaminase (TGase) activity between perivenous versus periportal regions of regenerating rat liver. Using the digitonin/collagenase perfusion technique, hepatocyte subpopulations were isolated from each region at various time points after partial hepatectomy. The amounts of DNA synthesis as well as the levels of TGase mRNA and activity in each subpopulation were measured. Although increased DNA synthesis was observed in both subpopulations with a peak at 24 h after partial hepatectomy, the amount of DNA synthesis in periportal hepatocytes (PPH) was twice as much as that in perivenous hepatocytes (PVH). In PVH, TGase activity peaked at 24 h after partial hepatectomy with a preceding increase in its mRNA expression at 12 h, whereas TGase activity in PPH at 24 h was one-half of that in PVH. As TGase is known to have a growth-arresting activity, our data indicate that relatively higher TGase activity in PVH at 24 h after partial hepatectomy might correlate with relatively lower DNA synthesis in this region compared to periportal region.
我们研究了再生大鼠肝脏的肝静脉周围区域与门静脉周围区域之间，DNA合成及转谷氨酰胺酶（TGase）活性的区域差异关系。采用洋地黄皂苷/胶原酶灌注技术，在部分肝切除术后的不同时间点，从每个区域分离出肝细胞亚群。测定了每个亚群中DNA合成量以及TGase mRNA水平和活性。虽然两个亚群的DNA合成均增加，且在部分肝切除术后24小时达到峰值，但门静脉周围肝细胞（PPH）的DNA合成量是肝静脉周围肝细胞（PVH）的两倍。在PVH中，TGase活性在部分肝切除术后24小时达到峰值，其mRNA表达在12小时前增加，而PPH在24小时时的TGase活性是PVH的一半。由于已知TGase具有生长抑制活性，我们的数据表明，部分肝切除术后24小时PVH中相对较高的TGase活性可能与该区域相比门静脉周围区域相对较低的DNA合成有关。
