Ohtake Yosuke, Maruko Akiko, Abe Shinya, Nagashima Tomomi, Fukumoto Manabu, Ohkubo Yasuhito
Department of Radiopharmacy, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan.
J Gastroenterol Hepatol. 2006 Nov;21(11):1726-30. doi: 10.1111/j.1440-1746.2006.04370.x.
The authors have recently demonstrated that there is inverse correlation between transglutaminase (TGase) activity and DNA synthesis in periportal hepatocytes (PPH) and perivenous hepatocytes (PVH) at 1 day after partial hepatectomy in rats. In order to ensure the involvement of TGase in the differential growth capacities between periportal and perivenous regions of regenerating liver, the aim of this study was to investigate the effect of retinoic acid, an inducer of TGase expression, on zonal differences of hepatocyte proliferation between PPH and PVH isolated from regenerating rat liver.
Regenerating liver was prepared by 70% partial hepatectomy. PPH and PVH subpopulations were isolated by the digitonin/collagenase perfusion technique. Cell cycle was evaluated for incorporation of BrdU into hepatocytes and detected by flow cytometric analysis. TGase activity was determined by incorporation of 14C-putrescine into dimethylcasein.
When retinoic acid was injected immediately after partial hepatectomy, TGase activity greatly increased in both PPH and PVH at 1 day after partial hepatectomy, and activity was higher in PPH than in PVH. DNA synthesis in both subpopulations did not increase 1 day after partial hepatectomy, with peaks of DNA synthesis shifting to 2 days, and synthesis was higher in PVH than in PPH.
These results suggest that TGase might be involved in differential growth capacities between periportal and perivenous regions of regenerating rat liver after partial hepatectomy.
作者最近证明，在大鼠部分肝切除术后1天，转谷氨酰胺酶（TGase）活性与门静脉周围肝细胞（PPH）和中央静脉周围肝细胞（PVH）中的DNA合成呈负相关。为了确定TGase是否参与再生肝脏门静脉周围和中央静脉周围区域不同的生长能力，本研究旨在探讨维甲酸（一种TGase表达诱导剂）对从再生大鼠肝脏分离的PPH和PVH之间肝细胞增殖的区域差异的影响。
通过70%部分肝切除术制备再生肝脏。采用洋地黄皂苷/胶原酶灌注技术分离PPH和PVH亚群。通过将BrdU掺入肝细胞来评估细胞周期，并通过流式细胞术分析进行检测。通过将14C-腐胺掺入二甲基酪蛋白来测定TGase活性。
部分肝切除术后立即注射维甲酸，在部分肝切除术后1天，PPH和PVH中的TGase活性均大幅增加，且PPH中的活性高于PVH。部分肝切除术后1天，两个亚群中的DNA合成均未增加，DNA合成峰值转移至2天，且PVH中的合成高于PPH。
这些结果表明，TGase可能参与部分肝切除术后再生大鼠肝脏门静脉周围和中央静脉周围区域不同的生长能力。
