Fernandes Karl J L, McKenzie Ian A, Mill Pleasantine, Smith Kristen M, Akhavan Mahnaz, Barnabé-Heider Fanie, Biernaskie Jeff, Junek Adrienne, Kobayashi Nao R, Toma Jean G, Kaplan David R, Labosky Patricia A, Rafuse Victor, Hui Chi-Chung, Miller Freda D
Department of Cancer Research, Hospital For Sick Children Research Institute, Ontario, Canada.
Nat Cell Biol. 2004 Nov;6(11):1082-93. doi: 10.1038/ncb1181.
A fundamental question in stem cell research is whether cultured multipotent adult stem cells represent endogenous multipotent precursor cells. Here we address this question, focusing on SKPs, a cultured adult stem cell from the dermis that generates both neural and mesodermal progeny. We show that SKPs derive from endogenous adult dermal precursors that exhibit properties similar to embryonic neural-crest stem cells. We demonstrate that these endogenous SKPs can first be isolated from skin during embryogenesis and that they persist into adulthood, with a niche in the papillae of hair and whisker follicles. Furthermore, lineage analysis indicates that both hair and whisker follicle dermal papillae contain neural-crest-derived cells, and that SKPs from the whisker pad are of neural-crest origin. We propose that SKPs represent an endogenous embryonic precursor cell that arises in peripheral tissues such as skin during development and maintains multipotency into adulthood.
干细胞研究中的一个基本问题是，培养的多能成体干细胞是否代表内源性多能前体细胞。在此，我们聚焦于皮肤源前体细胞（SKPs）来探讨这个问题，SKPs是一种从真皮中培养得到的成体干细胞，可产生神经和中胚层后代细胞。我们发现，SKPs来源于内源性成体真皮前体细胞，这些细胞具有与胚胎神经嵴干细胞相似的特性。我们证明，这些内源性SKPs在胚胎发育期间首先可从皮肤中分离出来，并且它们会持续存在至成年期，其生态位位于毛发和触须毛囊的乳头中。此外，谱系分析表明，毛发和触须毛囊的真皮乳头均含有神经嵴来源的细胞，并且来自触须垫的SKPs起源于神经嵴。我们提出，SKPs代表一种内源性胚胎前体细胞，它在发育过程中出现在诸如皮肤等外周组织中，并在成年期维持多能性。
