Perrault C M, Bray E J, Didier N, Ozaki C K, Tran-Son-Tay R
Department of Biomedical Engineering, University of Florida, Gainesville, Florida, USA.
Diabetologia. 2004 Oct;47(10):1722-6. doi: 10.1007/s00125-004-1524-2. Epub 2004 Oct 23.
AIMS/HYPOTHESIS: Clinical complications associated with diabetes may be related to altered physical properties of leucocytes. We used micropipette techniques to examine leucocyte rheology (specifically lymphocyte rheology) in the non-obese diabetic (NOD) mouse model of diabetes mellitus. We hypothesised that diabetes affects lymphocyte rheology, and specifically that lymphocyte membranes from diabetic mammals have a higher cortical tension than those from non-diabetic mammals.
Lymphocytes were isolated from diabetic and control mice. Lymphocyte deformation and activation were assessed with a micropipette apparatus. Cellular activation was assessed visually. Projection length into the micropipette during aspiration was used to calculate the viscosity of the cell. Recovery length following expulsion from the micropipette was used to derive the recovery time constant, which is the ratio of cortical tension : viscosity (T(o)/mu) for each cell. The cell cortical/surface tension was calculated from this ratio.
Of 692 control lymphocytes, 29% were spontaneously activated compared with 39% of 624 diabetic cells (p<0.06) and 31.5% of 315 non-diabetic NOD cells (p=0.14). Viscosity values for diabetic lymphocytes were equivalent to those for control cells (1345.12+/-1420.97 Pa.s vs 996.84+/-585.07 Pa.s, p=0.13). The average T(o)/micro value for diabetic lymphocytes (35.4+/-16.5x10(-6) cm/s) was significantly higher than that for control cells (24.8+/-11.3x10(-6) cm/s, p<0.03) and cells from non-diabetic NOD mice (26.3+/-9.0x10(-6) cm/s, p<0.005). The mean cortical tension values for diabetic and control cells were 4.7+/-2.3x10(-4) N/m and 2.8+/-0.7x10(-4) N/m respectively (p<0.003).
CONCLUSIONS/INTERPRETATION: Lymphocytes from diabetic mice tend to spontaneously activate. They have an equivalent cytoplasmic viscosity but a larger recovery time constant compared with cells from control mice. The results suggest that diabetic lymphocytes are stiffer than control cells.
目的/假设：与糖尿病相关的临床并发症可能与白细胞物理特性的改变有关。我们使用微量移液器技术研究糖尿病非肥胖糖尿病（NOD）小鼠模型中的白细胞流变学（特别是淋巴细胞流变学）。我们假设糖尿病会影响淋巴细胞流变学，具体而言，糖尿病哺乳动物的淋巴细胞膜比非糖尿病哺乳动物的具有更高的皮质张力。
从糖尿病小鼠和对照小鼠中分离淋巴细胞。使用微量移液器装置评估淋巴细胞变形和活化。通过视觉评估细胞活化。在抽吸过程中进入微量移液器的投影长度用于计算细胞的粘度。从微量移液器排出后的恢复长度用于得出恢复时间常数，其为每个细胞的皮质张力与粘度之比（T(o)/μ）。根据该比率计算细胞皮质/表面张力。
692个对照淋巴细胞中，29%自发活化，而624个糖尿病细胞中有39%（p<0.06），315个非糖尿病NOD细胞中有31.5%（p=0.14）。糖尿病淋巴细胞的粘度值与对照细胞相当（1345.12±1420.97Pa·s对996.84±585.07Pa·s，p=0.13）。糖尿病淋巴细胞的平均T(o)/μ值（35.4±16.5×10(-6)cm/s）显著高于对照细胞（24.8±11.3×10(-6)cm/s，p<0.03）和非糖尿病NOD小鼠的细胞（26.3±9.0×10(-6)cm/s，p<0.005）。糖尿病细胞和对照细胞的平均皮质张力值分别为4.7±2.3×10(-4)N/m和2.8±0.7×10(-4)N/m（p<0.003）。
结论/解读：糖尿病小鼠的淋巴细胞倾向于自发活化。与对照小鼠的细胞相比，它们具有相同的细胞质粘度但恢复时间常数更大。结果表明糖尿病淋巴细胞比对照细胞更硬。
