Tran-Son-Tay R, Needham D, Yeung A, Hochmuth R M
Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27706.
Biophys J. 1991 Oct;60(4):856-66. doi: 10.1016/S0006-3495(91)82119-1.
Experiments are performed in which a passive human neutrophil is deformed into an elongated "sausage" shape by aspirating it into a small glass pipette. When expelled from the pipette the neutrophil recovers its natural spherical shape in approximately 1 minute. This recovery process is analyzed according to a Newtonian, liquid-drop model in which a variational method is used to simultaneously solve the hydrodynamic equations for low Reynolds-number flow and the equations for membrane equilibrium with a constant membrane tension. The theoretical model gives a good fit to the experimental data for a ratio of membrane cortical tension to cytoplasmic viscosity of approximately 1.7 x 10(-5) cm/s (0.17 micron/s). However, when the cell is held in the pipette for only a short time period of 5 s or less, and then expelled, the cell undergoes an initial, rapid elastic rebound suggesting that the cell behaves in this instance as a Maxwell viscoelastic liquid rather than a Newtonian liquid with constant cortical tension.
进行了一些实验，在这些实验中，通过将被动的人类中性粒细胞吸入小玻璃吸管，使其变形为细长的“香肠”形状。当从中吸管排出时，中性粒细胞在大约1分钟内恢复其天然的球形。根据牛顿液滴模型分析这个恢复过程，在该模型中，使用变分方法同时求解低雷诺数流动的流体动力学方程和具有恒定膜张力的膜平衡方程。对于膜皮质张力与细胞质粘度之比约为1.7×10⁻⁵厘米/秒（0.17微米/秒）的情况，理论模型与实验数据拟合良好。然而，当细胞在吸管中仅保持5秒或更短的短时间，然后排出时，细胞会经历初始的快速弹性反弹，这表明细胞在这种情况下表现为麦克斯韦粘弹性液体，而不是具有恒定皮质张力的牛顿液体。
