大肠杆菌K-12中F质粒的非随机复制
Nonrandom F-plasmid replication in Escherichia coli K-12.
作者信息
Koppes L J
机构信息
Department of Microbiology, University of Uppsala Biomedical Center, Sweden.
出版信息
J Bacteriol. 1992 Apr;174(7):2121-3. doi: 10.1128/jb.174.7.2121-2123.1992.
Abstract
Both the autonomous and chromosomally integrated F plasmids were found to replicate in a nonrandom fashion after a density transfer from heavy medium ([13C]glucose, 15NH4) to light medium ([12C]glucose, 14NH4). The data are consistent with the hypothesis that both the chromosome and the F plasmid are replicated in a cell cycle-specific manner. Thus, these data support the proposal (J. D. Keasling, B. O. Palsson, and S. Cooper, J. Bacteriol. 173:2673-2680, 1991) that plasmids replicate in a cell cycle-specific manner.
摘要
在从重培养基（[¹³C]葡萄糖，¹⁵NH₄）到轻培养基（[¹²C]葡萄糖，¹⁴NH₄）进行密度转移后，发现自主型和染色体整合型F质粒均以非随机方式复制。这些数据与染色体和F质粒均以细胞周期特异性方式复制的假说一致。因此，这些数据支持了质粒以细胞周期特异性方式复制的提议（J. D. 基斯林、B. O. 帕尔松和S. 库珀，《细菌学杂志》173:2673 - 2680，1991年）。
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