Isobe Kazumasa, Kaneko Michio, Kaneko Setsuko, Nissato Sumiko, Nanmoku Toru, Takekoshi Kazuhiro, Okuda Yukichi, Kawakami Yasushi
Department of Clinical Pathology, Institute of Clinical Medicine, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki 305-8575, Japan.
Regul Pept. 2004 Dec 15;123(1-3):29-32. doi: 10.1016/j.regpep.2004.05.011.
Pituitary adenylate cyclase-activating polypeptide (PACAP), a member of the secretin/glucagon/vasoactive intestinal peptide family, induces the expression of catecholamine-synthesizing enzymes in adrenal medullary cells. In addition, PACAP and its receptor have been detected in human neuroblastoma tissues and cell lines, though it is not yet known whether PACAP enhances the expression of genes encoding catecholamine-synthesizing enzymes. To address this question, we analyzed PACAP, PACAP receptor and tyrosine hydroxylase (TH) mRNAs in neuroblastomas.
The levels of mRNA for PACAP and vasoactive intestinal peptide (VIP), as well as their receptors and the mRNA for TH were measured by RT-PCR or real-time PCR analysis.
VPAC1R mRNA was detected in all of 16 tissues and 3 cell lines that were examined, while VPAC2R mRNA was detected in 5 of 16 (31%) tissue and 2 of 3 cell lines. PAC1R mRNA was detected in 6 out of 16 (38%) tissues and none of 3 cell lines. mRNA expression of PACAP and TH were detected in many tissues (10/16 and 16/16, respectively). However, neither in tissues nor cell lines did PACAP mRNA expression correlate with TH mRNA expression.
Our findings suggest that PACAP is not involved in the regulation of expression of TH in neuroblastomas.
垂体腺苷酸环化酶激活多肽（PACAP）是促胰液素/胰高血糖素/血管活性肠肽家族的成员，可诱导肾上腺髓质细胞中儿茶酚胺合成酶的表达。此外，在人神经母细胞瘤组织和细胞系中已检测到PACAP及其受体，但尚不清楚PACAP是否能增强编码儿茶酚胺合成酶的基因的表达。为解决这个问题，我们分析了神经母细胞瘤中PACAP、PACAP受体和酪氨酸羟化酶（TH）的mRNA。
通过逆转录-聚合酶链反应（RT-PCR）或实时聚合酶链反应（real-time PCR）分析来测量PACAP和血管活性肠肽（VIP）的mRNA水平，以及它们的受体和TH的mRNA水平。
在所检测的16个组织和3个细胞系中，均检测到了VPAC1R mRNA，而在16个组织中的5个（31%）和3个细胞系中的2个检测到了VPAC2R mRNA。在16个组织中的6个（38%）检测到了PAC1R mRNA，而在3个细胞系中均未检测到。在许多组织中分别检测到了PACAP和TH的mRNA表达（分别为10/16和16/16）。然而，无论是在组织还是细胞系中，PACAP mRNA表达均与TH mRNA表达无相关性。
我们的研究结果表明，PACAP不参与神经母细胞瘤中TH表达的调节。
