照射后大鼠胸腺中PAC1受体的表达。

Expression of PAC1 receptor in rat thymus after irradiation.

作者信息

Tokuda Nobuko, Hamasaki Keiko, Mizutani Noriko, Adachi Yasuhiro, Sawada Tomoo, Funahashi Hisayuki, Shioda Seiji, Fukumoto Tetsuo

机构信息

Department of Human Science, Yamaguchi University School of Medicine, Ube 755-8505, Japan.

出版信息

Regul Pept. 2004 Dec 15;123(1-3):167-72. doi: 10.1016/j.regpep.2004.04.028.

DOI:10.1016/j.regpep.2004.04.028

PMID:15518908

Abstract

In the present work, PAC1-R (G-protein-coupled receptor specific for PACAP) was detected on cells in the normal thymus. Immunohistochemically PAC1-R was expressed strongly in stromal cells of the thymic medulla. Positive cells were also observed in the thymus of fetal and old adult rats. After 8 Gy irradiation to 9-week-old rats, PAC1-R expressions in the thymus decreased and almost recovered by day 21. The expression of PAC1-R mRNA was weak in the thymus and decreased further after irradiation. The expression almost recovered by day 28. Hip and hip/hop variants, which were not expressed in the normal thymus, were expressed in the thymus on days 3, 5 and 21 after irradiation. The expressions of IL-6 and IL-10 tended to increase initially after irradiation then decreased. Histologically, the thymic structures were destroyed on day 3 after irradiation and the thymus almost recovered by day 21. Thus PACAP is thought to be one of the important factors for cross-talk between cells involved in thymic regeneration.

摘要

在本研究中，在正常胸腺的细胞上检测到了PAC1-R（一种对垂体腺苷酸环化酶激活肽具有特异性的G蛋白偶联受体）。免疫组织化学显示，PAC1-R在胸腺髓质的基质细胞中强烈表达。在胎鼠和老年大鼠的胸腺中也观察到了阳性细胞。对9周龄大鼠进行8 Gy照射后，胸腺中PAC1-R的表达下降，到第21天几乎恢复。PAC1-R mRNA在胸腺中的表达较弱，照射后进一步下降。该表达在第28天几乎恢复。正常胸腺中未表达的Hip和Hip/hop变体在照射后第3天、第5天和第21天的胸腺中表达。白细胞介素-6和白细胞介素-10的表达在照射后最初趋于增加，然后下降。组织学上，照射后第3天胸腺结构被破坏，到第21天胸腺几乎恢复。因此，垂体腺苷酸环化酶激活肽被认为是参与胸腺再生的细胞间相互作用的重要因素之一。