Proano Cinthia E, Azar Dimitri T, Mocan Mehmet Cem, Redmond Robert W, Kochevar Irene E
Massachusetts Eye and Ear Infirmary and the Schepens Eye Research Institute, Massachusetts General Hospital, Boston, MA 02114, USA.
J Cataract Refract Surg. 2004 Nov;30(11):2420-4. doi: 10.1016/j.jcrs.2004.02.068.
To evaluate the benefit of photochemical keratodesmos (PKD) in acute wound closure of penetrating keratoplasty (PKP) corneal incisions in vivo.
Massachusetts General Hospital and Massachusetts Eye and Ear Infirmary, Boston, Massachusetts, USA.
Penetrating keratoplasty incisions were performed in both eyes of 6 New Zealand white rabbits, followed by application of 1.5 mM rose bengal dye at the wound edges. The incision in 1 eye of each rabbit was irradiated with neodymium:YAG laser light at 532 nm and fluence of 40 J/cm2; the contralateral control eye was untreated. Intraocular pressure at which fluid leaked at the edges (IOPL) was determined immediately after surgery.
The mean IOPL was 410 mm Hg +/- 70 (SD) in the PKD-treated eyes and 250 +/- 150 mm Hg in the unirradiated eyes. The difference was statistically significant by paired t test (P<.05).
Photochemical keratodesmos may be a useful adjunct to sutures for approximating PKP corneal incisions in the immediate postoperative period. This process does not induce high temperature, and thus denaturation can be avoided and structural integrity restored.
评估光化学角膜固定术（PKD）在体内穿透性角膜移植术（PKP）角膜切口急性伤口闭合中的作用。
美国马萨诸塞州波士顿的麻省总医院和麻省眼耳医院。
对6只新西兰白兔的双眼进行穿透性角膜移植切口，然后在伤口边缘应用1.5 mM孟加拉玫瑰红染料。每只兔子的一只眼睛的切口用波长532 nm、能量密度40 J/cm²的钕:钇铝石榴石激光照射；对侧对照眼不进行处理。术后立即测定伤口边缘出现液体渗漏时的眼压（IOPL）。
PKD治疗组眼睛的平均IOPL为410 mmHg±70（标准差），未照射组眼睛为250±150 mmHg。配对t检验显示差异有统计学意义（P<0.05）。
光化学角膜固定术可能是术后早期用于近似PKP角膜切口缝线的有用辅助方法。该过程不会诱导高温，因此可以避免变性并恢复结构完整性。
