壳聚糖黏附膜光化学组织黏合。
Photochemical tissue bonding with chitosan adhesive films.
机构信息
School of Biomedical and Health Sciences, School of Medicine, University of Western Sydney, NSW, Australia.
出版信息
Biomed Eng Online. 2010 Sep 8;9:47. doi: 10.1186/1475-925X-9-47.
BACKGROUND
Photochemical tissue bonding (PTB) is a promising sutureless technique for tissue repair. PTB is often achieved by applying a solution of rose bengal (RB) between two tissue edges, which are irradiated by a green laser to crosslink collagen fibers with minimal heat production. In this study, RB has been incorporated in chitosan films to create a novel tissue adhesive that is laser-activated.
METHODS
Adhesive films, based on chitosan and containing 0.1 wt% RB were manufactured and bonded to calf intestine by a solid state laser (λ = 532 nm, Fluence110 J/cm2, spot size~0.5 cm). A single-column tensiometer, interfaced with a personal computer, tested the bonding strength. K-type thermocouples recorded the temperature (T) at the adhesive-tissue interface during laser irradiation. Human fibroblasts were also seeded on the adhesive and cultured for 48 hours to assess cell growth.
RESULTS
The RB-chitosan adhesive bonded firmly to the intestine with adhesion strength of 15 ± 2 kPa, (n = 31). The adhesion strength dropped to 0.5 ± 0.1 (n = 8) kPa when the laser was not applied to the adhesive. The average temperature of the adhesive increased from 26°C to 32°C during laser exposure. Fibroblasts grew confluent on the adhesive without morphological changes.
CONCLUSION
A new biocompatible chitosan adhesive has been developed that bonds photochemically to tissue with minimal temperature increase.
背景
光化学组织黏合（PTB）是一种有前途的无缝合技术，可用于组织修复。PTB 通常通过在两个组织边缘之间施加玫瑰红 Bengal（RB）溶液来实现，然后用绿光激光照射以最小的产热交联胶原纤维。在这项研究中，RB 已被掺入壳聚糖薄膜中，以创建一种新型的激光激活组织粘合剂。
方法
制造了基于壳聚糖且含有约 0.1wt%RB 的粘合膜，并通过固态激光器（λ=532nm，Fluence110J/cm2，光斑尺寸0.5cm）将其粘合到小牛肠上。单柱张力计与个人计算机接口，测试粘合强度。K 型热电偶记录激光照射过程中粘合-组织界面的温度（T）。还将人成纤维细胞播种在粘合剂上，并培养 48 小时以评估细胞生长。
结果
RB-壳聚糖粘合剂与肠牢固地粘合在一起，粘合强度为 15±2kPa（n=31）。当未将激光应用于粘合剂时，粘合强度降至 0.5±0.1kPa（n=8）。在激光照射过程中，粘合剂的平均温度从 26°C 升高到 32°C。成纤维细胞在粘合剂上生长并融合，形态无变化。
结论
已经开发出一种新的生物相容性壳聚糖粘合剂，它可以通过光化学与组织粘合，并且温度升高最小。
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