Ferrer Consuelo, Ruiz-Moreno José M, Rodríguez Alejandra, Montero Javier, Alió Jorge L
Departamento de Biología Molecular, VISSUM, Instituto Oftalmológico de Alicante, Alicante, Spain.
J Cataract Refract Surg. 2004 Nov;30(11):2441-4. doi: 10.1016/j.jcrs.2004.04.056.
A 72-year-old man with chronic endophthalmitis who received steroid treatment for 3 months came to our center. Sterile endophthalmitis after cataract extraction had been diagnosed. Aqueous samples including smears, classic cultures, and polymerase chain reaction were taken for microbiological study. Amplified DNA was sequenced to identify the pathogen. Polymerase chain reaction amplification was positive for bacteria. Sequence analysis showed Corynebacterium macginleyi as the causal agent in 48 hours. The culture and smear stains from the ocular samples were negative. The patient was successfully treated with vancomycin. Polymerase chain reaction and subsequent DNA-typing were useful in detecting the microorganisms that caused the chronic endophthalmitis.
一名患有慢性眼内炎的72岁男性，接受了3个月的类固醇治疗后前来我们中心。此前已诊断为白内障摘除术后无菌性眼内炎。采集了包括涂片、传统培养和聚合酶链反应的房水样本进行微生物学研究。对扩增的DNA进行测序以鉴定病原体。聚合酶链反应扩增显示细菌呈阳性。序列分析显示麦氏棒状杆菌是48小时内的病原体。眼部样本的培养和涂片染色均为阴性。患者接受万古霉素治疗成功。聚合酶链反应及后续的DNA分型有助于检测引起慢性眼内炎的微生物。
