通过安培传感器监测，用硫氰酸钾从S-亚硝基半胱氨酸中选择性释放一氧化氮。

Selective liberation of NO from S-nitrosocysteine with potassium thiocyanate, as monitored by an amperometric sensor.

作者信息

Alberto Palmerini Carlo, Marmottini Fabio, Arienti Giuseppe, Palombari Roberto

机构信息

Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Via del Giochetto, 06127 Perugia, Italy.

出版信息

Arch Biochem Biophys. 2004 Dec 1;432(1):37-40. doi: 10.1016/j.abb.2004.09.014.

DOI:10.1016/j.abb.2004.09.014

PMID:15519294

Abstract

S-Nitrosocysteine (CysNO) releases either NO (in the presence of divalent cations) or NO+ (in the presence of chelating agents). NO+ is then transferred to peptides or protein SH groups to form high-mass nitrosothiols. The aim of this work was the development of a specific reaction between thiocyanate (SCN-) and CysNO. This reaction selectively liberates NO from CysNO in the presence of high-mass nitrosothiols. Free NO is measured with an amperometric sensor. We examine with this system the transnitrosylation reaction between CysNO and BSA at low molecular ratios and could assay nitrites, SNO-BSA, and CysNO in the incubation mixture without any preliminary purification steps.

摘要

S-亚硝基半胱氨酸（CysNO）在二价阳离子存在下释放一氧化氮（NO），或在螯合剂存在下释放硝酰阳离子（NO⁺）。然后，NO⁺转移至肽或蛋白质的巯基上，形成高质量的亚硝基硫醇。本研究的目的是开发硫氰酸盐（SCN⁻）与CysNO之间的特异性反应。该反应在高质量亚硝基硫醇存在下从CysNO中选择性释放NO。游离的NO用安培传感器进行测定。我们用该系统研究了低分子比下CysNO与牛血清白蛋白（BSA）之间的亚硝基化反应，并且无需任何初步纯化步骤就能测定孵育混合物中的亚硝酸盐、SNO-BSA和CysNO。