检测樱桃卷叶病毒核桃株系的不同方法评估
An appraisal of different methods for the detection of the walnut strain of cherry leafroll virus.
作者信息
Borja M J, Ponz F
机构信息
CIT-INIA, Dpto. Protección Vegetal, Crta. La Coruña, Madrid, Spain.
出版信息
J Virol Methods. 1992 Jan;36(1):73-83. doi: 10.1016/0166-0934(92)90158-a.
Abstract
Three methods were evaluated for the detection of cherry leafroll virus: ELISA, dot-blot and reverse transcriptional polymerase chain reaction (RT-PCR). Dot-blot and RT-PCR were carried out in crude plant extracts without any further RNA purification. Dot-blot hybridization using a 32P-labelled DNA probe was as sensitive as previously reported ELISA results for cherry leafroll virus detection. The most sensitive method was RT-PCR, which amplified a specific fragment of 448 bp from the 3' untranslated region of both viral genomic RNAs. RT-PCR was used to detect cherry leafroll virus in infected walnut buds and twigs.
摘要
对三种检测樱桃卷叶病毒的方法进行了评估
酶联免疫吸附测定（ELISA）、斑点杂交和逆转录聚合酶链反应（RT-PCR）。斑点杂交和RT-PCR在未经进一步RNA纯化的粗植物提取物中进行。使用32P标记的DNA探针进行的斑点杂交检测樱桃卷叶病毒的灵敏度与先前报道的ELISA结果相同。最灵敏的方法是RT-PCR，它从两种病毒基因组RNA的3'非翻译区扩增出一个448 bp的特定片段。RT-PCR用于检测受感染核桃芽和嫩枝中的樱桃卷叶病毒。
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