使用固相萃取、液相色谱/质谱联用及组类比内标法测定医院污水中的抗生素物质。
Determination of antibiotic substances in hospital sewage water using solid phase extraction and liquid chromatography/mass spectrometry and group analogue internal standards.
作者信息
Lindberg Richard, Jarnheimer Per-Ake, Olsen Björn, Johansson Magnus, Tysklind Mats
机构信息
Department of Chemistry, Environmental Chemistry, Umeå University, SE-907 87 Umeå, Sweden.
出版信息
Chemosphere. 2004 Dec;57(10):1479-88. doi: 10.1016/j.chemosphere.2004.09.015.
A method for determination of antibiotics in hospital sewage water has been developed and validated. Analogue internal standards for fluoroquinolones, sulfonamides, trimethoprim, beta-lactams (penicillins and cephalosporins), nitroimidazoles and tetracyclines were successfully used for calibration and shown to generally improve precision compared to external calibration. Matrix components caused ion suppression/enhancement effects during the MS detection for all analytes studied. Two effects were observed: general suppression and short-term variations in the MS response. In the hospital sewage water large temporal variations in the analyte concentration were observed during the course of the sampling period (seven grab samples in 13 h). Analyte concentrations varied within the following ranges (in microg l(-1)): ciprofloxacin, 3.6-101.0; metronidazole, 0.1-90.2; sulfamethoxazole, 0.4-12.8; ofloxacin, 0.2-7.6; trimethoprim, 0.6-7.6; and doxycycline, 0.6-6.7.
已开发并验证了一种测定医院污水中抗生素的方法。氟喹诺酮类、磺胺类、甲氧苄啶、β-内酰胺类（青霉素类和头孢菌素类）、硝基咪唑类和四环素类的类似物内标已成功用于校准，并且与外标法相比，通常显示出更高的精密度。在所研究的所有分析物的质谱检测过程中，基质成分均引起离子抑制/增强效应。观察到两种效应：一般抑制和质谱响应的短期变化。在采样期间（13小时内采集7个瞬时水样），医院污水中分析物浓度存在较大的时间变化。分析物浓度在以下范围内变化（以μg l(-1)计）：环丙沙星，3.6 - 101.0；甲硝唑，0.1 - 90.2；磺胺甲恶唑，0.4 - 12.8；氧氟沙星，0.2 - 7.6；甲氧苄啶，0.6 - 7.6；强力霉素，0.6 - 6.7。
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