Fernandes Cathy, Liu Lin, Paya-Cano José L, Gregorová Sona, Forejt Jirí, Schalkwyk Leonard C
Social, Genetic and Developmental Psychiatry Centre, PO 82, Institute of Psychiatry, King's College London, De Crespigny Park, London SE5 8AF, UK. c.
Behav Genet. 2004 Nov;34(6):621-30. doi: 10.1007/s10519-004-5589-y.
PWD/Ph is an inbred mouse strain derived from wild mice trapped in central Czech Republic. These mice are of the Mus musculus musculus subspecies, whose ancestors separated from those of Mus musculus domesticus about one million years ago. There is a high degree of variation in the genomic sequence and a wide range of phenotypes between PWD/Ph and standard laboratory inbred mouse strains, the genomes of which are principally Mus musculus domesticus in origin, making PWD/Ph mice an useful resource for complex trait research. As a first step in taking advantage of this resource, a preliminary characterization of the behavior of PWD/Ph mice was performed. Groups of 10 PWD/Ph and C57BL/6J male mice were tested in the open field, novel object exploration task and Morris water maze. PWD/Ph were marginally more anxious than C57BL/6J mice in the open field but subsequently displayed much higher levels of exploration and lower anxiety than C57BL/6J mice following introduction of a novel object. As C57BL/6J itself is rated as highly explorative among classical inbred strains, PWD/Ph probably represents an extreme among mouse strains for this specific behavior. PWD/Ph and C57BL/6J mice differed in their water escape training profiles in the Morris water maze, perhaps reflecting different motivational factors. However, there were no differences in overall cognitive ability (spatial learning) as both groups learned to find the hidden platform and performed equally well when the location of the platform was changed. This is the first quantification of the behavior of PWD/Ph mice and the results are promising for the potential of the consomic panel currently being generated with PWD/Ph and C57BL/6J as a tool for the molecular dissection of behavior.
PWD/Ph是一种近交系小鼠，源自于在捷克共和国中部捕获的野生小鼠。这些小鼠属于小家鼠（Mus musculus musculus）亚种，其祖先大约在一百万年前与家鼠（Mus musculus domesticus）的祖先分离。PWD/Ph与标准实验室近交系小鼠品系之间在基因组序列上存在高度变异，并且在表型上有广泛差异，标准实验室近交系小鼠品系的基因组主要起源于家鼠，这使得PWD/Ph小鼠成为复杂性状研究的有用资源。作为利用这一资源的第一步，对PWD/Ph小鼠的行为进行了初步表征。将10只PWD/Ph雄性小鼠和10只C57BL/6J雄性小鼠分组，在旷场实验、新物体探索任务和莫里斯水迷宫中进行测试。在旷场实验中，PWD/Ph小鼠比C57BL/6J小鼠略显焦虑，但在引入新物体后，PWD/Ph小鼠随后表现出比C57BL/6J小鼠更高的探索水平和更低的焦虑程度。由于C57BL/6J本身在经典近交系中被评为具有高度探索性，PWD/Ph在这种特定行为方面可能代表了小鼠品系中的一个极端。PWD/Ph小鼠和C57BL/6J小鼠在莫里斯水迷宫中的水逃避训练情况有所不同，这可能反映了不同的动机因素。然而，两组在总体认知能力（空间学习）方面没有差异，因为两组都学会了找到隐藏平台，并且在平台位置改变时表现相当。这是对PWD/Ph小鼠行为的首次量化，其结果对于目前正在构建的以PWD/Ph和C57BL/6J为工具进行行为分子剖析的染色体代换系的潜力来说很有前景。
