Department of Pediatrics, Medical University of Vienna, Austria.
Hippocampus. 2011 Jul;21(7):714-23. doi: 10.1002/hipo.20788. Epub 2010 Apr 13.
There is a significant strain-dependent performance in the Morris water maze (MWM), a paradigm for the evaluation of spatial memory. In contrast, information on molecular differences that may be responsible for differences in spatial memory performance is limited. The aim of the study was therefore to investigate differences in hippocampal protein levels in three groups with different performance in the MWM. C57BL/6J (inbred laboratory strain), PWD/PhJ (inbred strain derived from wild animals of Mus musculus), and Apodemus sylvaticus (AS, genus Apodemus) mice were used for the experiments. Proteins from hippocampi, obtained from a behavioral study on these animals, were extracted and run on two-dimensional gel electrophoresis. Proteins spots were quantified, and spots with significantly different levels were identified by mass spectrometry using an ion trap. A series of 49 proteins from different pathways and cascades (signaling, neuronal network, protein synthesis, secretion and degradation, and antioxidant system; intermediary, fat, and carbohydrate metabolism) were significantly different among hippocampi at the stringent statistical level of P ≤ 0.001. These findings are paralleled by differences in the spatial navigation abilities between the strains within the species Mus musculus (C57BL/6 vs. PWD/PhJ) and between the genera Mus and Apodemus. As shown previously, AS learned the task in the MWM and showed good memory retention when tested at the probe trial (day 12), whereas C57BL/6J learned the task, but failed at the probe trial at day 12 as well as PWD/PhJ that failed to learn the task and failed at the probe trial at day 12. A list of above-mentioned proteins were different between PWD/PhJ with bad and AS with good memory retention and may therefore be related to performance in the MWM and thus to spatial memory formation. The experimental approach, however, does not allow discriminating between differences in protein levels a priori and different protein levels induced by the MWM testing. © 2010 Wiley-Liss, Inc.
在水迷宫（MWM）中存在显著的依赖品系的表现，这是一种用于评估空间记忆的范式。相比之下，关于可能导致空间记忆表现差异的分子差异的信息是有限的。因此，本研究的目的是研究在MWM 中表现不同的三个组的海马蛋白水平的差异。C57BL/6J（近交实验室品系）、PWD/PhJ（源自野生动物 Mus musculus 的近交品系）和 Apodemus sylvaticus（AS，属 Apodemus）的小鼠被用于实验。从这些动物的行为研究中提取海马蛋白，并在二维凝胶电泳上运行。定量蛋白质斑点，并用离子阱通过质谱法鉴定具有显著不同水平的斑点。来自不同途径和级联（信号转导、神经元网络、蛋白质合成、分泌和降解以及抗氧化系统；中间代谢物、脂肪和碳水化合物代谢）的一系列 49 种蛋白质在严格的统计学水平 P ≤ 0.001 时在海马中具有显著差异。这些发现与 Mus musculus（C57BL/6 与 PWD/PhJ）种内品系之间以及 Mus 和 Apodemus 属之间的空间导航能力差异相平行。如前所述，AS 在 MWM 中学习任务，并在探针试验（第 12 天）中表现出良好的记忆保留，而 C57BL/6J 学习任务，但在第 12 天的探针试验中失败，以及未能学习任务并在第 12 天的探针试验中失败的 PWD/PhJ。在记忆保留良好的 AS 与记忆保留较差的 PWD/PhJ 之间，上述蛋白质列表存在差异，因此可能与 MWM 中的表现和空间记忆形成有关。然而，实验方法不能预先区分蛋白质水平的差异和 MWM 测试引起的不同蛋白质水平。2010 年 Wiley-Liss，Inc.
