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来自波兰米德维湖的多形饰贝(斑马贻贝)的细胞遗传学特征

Cytogenetic characterization of the Zebra Mussel Dreissena polymorpha (Pallas) from Miedwie Lake, Poland.

作者信息

Boroń Alicja, Woźnicki Paweł, Skuza Lidia, Zieliński Roman

机构信息

Department of Zoology, Faculty of Biology, Warmia and Mazury University in Olsztyn, 10-718 Olsztyn, Poland.

出版信息

Folia Biol (Krakow). 2004;52(1-2):33-8.

Abstract

Cytogenetic characterization of D. polymorpha was carried out using banding techniques such as C-banding, fluorochrome CMA3 and silver nitrate treatment. The diploid chromosome number of both investigated D. polymorpha forms (typical and albinotic) was the same 2n = 32 (NF = 56). The karyotype consisted of 5 pairs of metacentric, 7 pairs of submetacentric and four pairs of subtelo-acrocentric chromosomes. Ag-NORs were located in the telomeric position on the largest subtelo-acrocentric chromosome pair. C banding patterns indicate many sites of constitutive heterochromatin mainly located in the telomeric regions and interstitially in some chromosomes. CMA3-sites were observed in almost all chromosomes; apart from the Ag-NORs sites, they were located terminally on the chromosome arms and interstitially on three chromosome pairs. Sixteen chromosomes could be counted at the diakinesis stage of meiosis. No differences in banding chromosome patterns were found neither between both analyzed forms of D. polymorpha nor between males and females.

摘要

利用C带、荧光染料CMA3和硝酸银处理等显带技术对多形饰贝进行了细胞遗传学特征分析。所研究的两种多形饰贝形态(典型型和白化型)的二倍体染色体数均为2n = 32(NF = 56)。核型由5对中着丝粒染色体、7对亚中着丝粒染色体和4对近端着丝粒染色体组成。银染核仁组织区位于最大的近端着丝粒染色体对的端粒位置。C带模式表明组成型异染色质的许多位点主要位于端粒区域,在一些染色体上也有居间分布。在几乎所有染色体上都观察到了CMA3位点;除了银染核仁组织区位点外,它们位于染色体臂的末端以及三对染色体的居间位置。在减数分裂终变期可以计数16条染色体。在所分析的两种多形饰贝形态之间以及雌雄之间,在染色体带型模式上均未发现差异。

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