Genomic hybridization in interspecific hybrids of scallops (Bivalvia, Pectinidae) and localization of the satellite DNA , and the vertebrate telomeric sequences (TTAGGG)n on chromosomes of scallop (Jones & Preston, 1904).

Mitotic chromosome preparations of the interspecific hybrids (Jones & Preston, 1904) × (Jay, 1857), × (Lamarck, 1819) and × (Reeve, 1852) were used to compare two different scallop genomes in a single slide. Although genomic hybridization (GISH) using genomic DNA from each scallop species as probe painted mitotic chromosomes of the interspecific hybrids, the painting results were not uniform; instead it showed species-specific distribution patterns of fluorescent signals among the chromosomes. The most prominent GISH-bands were mainly located at centromeric or telomeric regions of scallop chromosomes. In order to illustrate the sequence constitution of the GISH-bands, the satellite sequences of and the vertebrate telomeric (TTAGGG) sequences were used to map mitotic chromosomes of by fluorescence hybridization (FISH). The results indicated that the GISH-banding pattern presented by the chromosomes of is mainly due to the distribution of the satellite DNA, therefore suggesting that the GISH-banding patterns found in the other three scallops could also be the result of the chromosomal distribution of other species-specific satellite DNAs.