Gunji Wataru, Kai Takahito, Sameshima Eiko, Iizuka Naomi, Katagi Hiroaki, Utsugi Takahiko, Fujimori Fumihiro, Murakami Yasufumi
Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
Biochem Biophys Res Commun. 2004 Dec 3;325(1):265-75. doi: 10.1016/j.bbrc.2004.10.025.
We manufactured a highly sensitive oligonucleotide microarray system comprised entirely of transcription regulatory factors (a TF oligo microarray) in order to comprehensively analyze the expression profiles of transcription factors in mice. We compared the expression profiles of transcription regulatory factors in mouse embryonic stem (ES) cells and ES-differentiated cells by using this TF oligo microarray, a cDNA microarray, a GeneChip system, and quantitative RT-PCR. The TF oligo microarray was able to comprehensively analyze the expression profile of transcription regulatory factors. In addition, we used the manufactured TF oligo microarray to analyze the expression patterns of transcriptional regulatory factors during the formation of embryoid bodies. The TF array was able to reveal the chronologic expression profile of transcription regulatory factors involved in embryogenesis or the maintenance of pluripotency in ES cells.
为了全面分析小鼠转录因子的表达谱，我们制造了一种完全由转录调节因子组成的高灵敏度寡核苷酸微阵列系统（转录因子寡核苷酸微阵列）。我们使用这种转录因子寡核苷酸微阵列、cDNA微阵列、基因芯片系统和定量逆转录聚合酶链反应，比较了小鼠胚胎干细胞（ES细胞）和ES分化细胞中转录调节因子的表达谱。转录因子寡核苷酸微阵列能够全面分析转录调节因子的表达谱。此外，我们使用制造的转录因子寡核苷酸微阵列分析胚状体形成过程中转录调节因子的表达模式。转录因子阵列能够揭示参与胚胎发生或ES细胞多能性维持的转录调节因子的时间表达谱。
