Lee Jungsoo, Jee Changhoon, Song Hyun-Ok, Bandyopadhyay Jaya, Lee Jin Il, Yu Jae-Ran, Lee Jiyeon, Park Byung-Jae, Ahnn Joohong
Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, South Korea.
J Mol Biol. 2004 Nov 19;344(2):585-95. doi: 10.1016/j.jmb.2004.09.050.
Ca(2+)/calmodulin-dependent calcineurin has been shown to have important roles in various Ca(2+) signaling pathways. We have previously reported that cnb-1(jh103) mutants, null mutants of a regulatory B subunit, displayed pleiotropic defects including uncoordinated movement and delayed egg laying in Caenorhabditis elegans. Interestingly, gain-of-function mutants of a catalytic A subunit showed exactly opposite phenotypes to those of cnb-1(null) mutants providing an excellent genetic model to define calcium-mediated signaling pathway at the organism level. Furthermore, calcineurin is also important for normal cuticle formation, which is required for maintenance of normal body size in C.elegans. Genetic interactions between tax-6 and several mutants including egl-30 and egl-10, which are known to be involved in G-protein signaling pathways suggest that calcineurin indeed regulates locomotion and serotonin-mediated egg laying through goa-1(Goalpha) and egl-30(Gqalpha). Our results indicate that, along with CaMKII, calcineurin regulates G-protein-coupled phosphorylation signaling pathways in C.elegans.
已证明钙调神经磷酸酶（Ca(2+)/钙调蛋白依赖性）在各种Ca(2+)信号通路中发挥重要作用。我们之前报道过，cnb-1(jh103)突变体（一种调节性B亚基的缺失突变体）在秀丽隐杆线虫中表现出多效性缺陷，包括不协调运动和产卵延迟。有趣的是，催化性A亚基的功能获得性突变体表现出与cnb-1(缺失)突变体完全相反的表型，为在生物体水平定义钙介导的信号通路提供了一个出色的遗传模型。此外，钙调神经磷酸酶对于正常表皮形成也很重要，而这是秀丽隐杆线虫维持正常体型所必需的。tax-6与包括egl-30和egl-10在内的几个已知参与G蛋白信号通路的突变体之间的遗传相互作用表明，钙调神经磷酸酶确实通过goa-1（Goα）和egl-30（Gqα）调节运动和5-羟色胺介导的产卵。我们的结果表明，与CaMKII一起，钙调神经磷酸酶在秀丽隐杆线虫中调节G蛋白偶联的磷酸化信号通路。
