Department of Life Science, BK21 (Life Science for Global Warming Team), College of Natural Sciences, Hanyang University, Seoul 133-791, Korea.
BMB Rep. 2011 Feb;44(2):96-101. doi: 10.5483/BMBRep.2011.44.2.96.
Calcineurin is a serine/threonine protein phosphatase controlled by Ca(2+) and calmodulin that has been implicated in various signaling pathways. Previously, we reported that calcineurin regulates coelomocyte endocytosis in Caenorhabditis elegans. So far, simple and powerful in vivo approaches have been developed to study various endocytic processes in C. elegans. Using these in vivo assays, we further analyzed the endocytic phenotypes of calcineurin mutants. We observed that the calcineurin mutants were defective in apical endocytosis in the intestine as well as synaptic vesicle recycling in the nerve cord. However, we found that calcineurin mutants displayed normal receptor-mediated endocytosis in oocytes. Therefore, our results suggest that calcineurin may regulate specific sets of endocytic processes in nematode.
钙调神经磷酸酶是一种丝氨酸/苏氨酸蛋白磷酸酶，受 Ca(2+)和钙调蛋白调控，与多种信号通路有关。此前，我们报道钙调神经磷酸酶调控秀丽隐杆线虫的体腔细胞内吞作用。目前，已经开发出了简单而强大的活体方法来研究秀丽隐杆线虫中的各种内吞作用过程。利用这些活体测定法，我们进一步分析了钙调神经磷酸酶突变体的内吞表型。我们观察到钙调神经磷酸酶突变体在肠道中的顶质内吞作用以及神经索中的突触小泡再循环过程中存在缺陷。然而，我们发现钙调神经磷酸酶突变体在卵母细胞中表现出正常的受体介导的内吞作用。因此，我们的结果表明钙调神经磷酸酶可能在线虫中调控特定的内吞作用过程。
