Yuan Jie, Chen Min-Yi, Shao Peng, Zhou Hui, Chen Yue-Qin, Qu Liang-Hu
Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275, China.
FEMS Microbiol Lett. 2004 Nov 15;240(2):163-70. doi: 10.1016/j.femsle.2004.09.030.
Population structures and genetic diversity of the small eukaryotic plankton from the coastal waters of the Nansha Islands in China were investigated. Two genes libraries using 18S rDNA of the marine small eukaryotes were constituted, and 323 clones were identified within alveolates (more than 43%), acanthareas, viridiplantaes, and stramenopiles. Many novel clones were detected in the two libraries, including two groups of alveolates and two clades related to both acanthareas and polycystineas. Several sequences unrelated to any other known eukaryotes may represent early branches in the phylogenetic tree. Our results reveal that there is a high diversity and abundance of small eukaryotes in the marine regions of China.
对中国南沙群岛沿海水域小型真核浮游生物的种群结构和遗传多样性进行了调查。构建了两个使用海洋小型真核生物18S rDNA的基因文库，在肺泡虫（超过43%）、棘阿米巴原虫、绿藻和不等鞭毛类中鉴定出323个克隆。在这两个文库中检测到许多新的克隆，包括两组肺泡虫以及与棘阿米巴原虫和多囊虫都相关的两个进化枝。一些与任何其他已知真核生物无关的序列可能代表了系统发育树中的早期分支。我们的结果表明，中国海洋区域小型真核生物具有高度的多样性和丰富度。
