Li Jishan, Wang Hua, Deng Ting, Wu Zhaoyang, Shen Guoli, Yu Ruqin
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Biosens Bioelectron. 2004 Nov 1;20(4):841-7. doi: 10.1016/j.bios.2004.03.023.
A capacitance immunosensor based on a plasma-polymerized ethylenediamine film (PPEF) has been developed. The resulting PPEF is studied with scanning electrode micrograph (SEM), IR reflection spectrum and cyclic voltammetry. SEM and IR reflection spectrum showed that the plasma-polymerized film (PPF) formed on the gold electrode surface is quite homogeneous, flat, nonporous and contains plenty of free-reacted -NH2. Moreover, cyclic voltammetry showed that the hexacyanoferrate redox reactions were blocked well by the formed PPF, that is to say, the formed PPF has excellent insulating characteristics. To investigate its applicability for capacitive immunosensing, goat-anti-human IgG antibody (IgGAb) was coupled to the PPF-coated gold electrode surface via glutaraldehyde (GA) to form an immunoglobulin G (IgG) probe. Alternating current (ac) impedance and capacitance measurement were used in the immunoassay. The experiment results show that the PPEF is applicable to form insulating layer of capacitive immunosensors.
一种基于等离子体聚合乙二胺薄膜（PPEF）的电容免疫传感器已被开发出来。通过扫描电极显微镜（SEM）、红外反射光谱和循环伏安法对所得的PPEF进行了研究。SEM和红外反射光谱表明，在金电极表面形成的等离子体聚合薄膜（PPF）相当均匀、平整、无孔且含有大量自由反应的 -NH2。此外，循环伏安法表明，形成的PPF能很好地阻断六氰合铁酸盐氧化还原反应，也就是说，形成的PPF具有优异的绝缘特性。为了研究其在电容免疫传感中的适用性，通过戊二醛（GA）将山羊抗人IgG抗体（IgGAb）偶联到涂有PPF的金电极表面，以形成免疫球蛋白G（IgG）探针。在免疫测定中使用了交流（ac）阻抗和电容测量。实验结果表明，PPEF适用于形成电容免疫传感器的绝缘层。
