Pliquett U, Gusbeth C
Faculty of Chemistry, Physical and Biophysical Chemistry, University of Bielefeld, D-33615 Bielefeld, Germany.
Bioelectrochemistry. 2004 Dec;65(1):27-32. doi: 10.1016/j.bioelechem.2004.05.005.
The electroporative effect on the stratum corneum (SC) is highly localized. However, the fractional area for the transport of small ions and larger ionic species differs considerably during and after high voltage (HV) application. Electroporation of SC creates new aqueous pathways, accessible for small ions, such as Cl(-) and Na(+) ions. The pores are distributed across the skin surface yielding a fractional area for current flow during electroporation of up to 0.1%. An increased permeability after high voltage application persists within a fractional area on the order of 10(-3)%. The permeabilization of SC for larger, charged molecules (M > 200 g/mol) involves Joule heating and a phase transition of the long chain sphingolipids within local transport regions (LTR). The transport area for these molecules (approximately 10(-3)%) changes only negligibly after high voltage application.
电穿孔对角质层（SC）的作用高度局限。然而，在施加高压（HV）期间及之后，小离子和较大离子种类的转运分数面积差异很大。角质层的电穿孔会产生新的水性通道，可供诸如Cl(-)和Na(+)等离子的小离子通过。这些孔分布在皮肤表面，在电穿孔期间产生的电流流动分数面积高达0.1%。施加高压后增加的渗透性在约10(-3)%的分数面积内持续存在。对于较大的带电分子（M > 200 g/mol），角质层的通透化涉及焦耳热以及局部转运区域（LTR）内长链鞘脂的相变。施加高压后，这些分子的转运面积（约10(-3)%）变化可忽略不计。
