Volpi Nicola
Department of Biologia Animale, University of Modena and Reggio Emilia, Via Campi 213/d, 41100 Modena, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Nov 25;811(2):253-6. doi: 10.1016/j.jchromb.2004.09.004.
The high-performance capillary electrophoresis (HPCE) (electrokinetic chromatography with sodium dodecyl sulphate) technique was applied to the extraction and purification process of the K4 polysaccharide from cultured bacteria in several stages. HPCE proved to be a technique with high resolution and sensitivity in analyzing K4 polysaccharide during its purification, in particular by using a strong anion-exchange resin. This is of paramount importance to monitor the product during the extraction and purification process or to test the purity of the final product. Furthermore, HPCE is able to verify that the extraction and purification process adopted is not carried out under drastic conditions capable of inducing fructose removal from the polysaccharide backbone.
高效毛细管电泳（HPCE）（十二烷基硫酸钠电动色谱法）技术被应用于从培养细菌中提取和纯化K4多糖的多个阶段。事实证明，HPCE是一种在K4多糖纯化过程中分析其具有高分辨率和灵敏度的技术，特别是使用强阴离子交换树脂时。这对于在提取和纯化过程中监测产品或测试最终产品的纯度至关重要。此外，HPCE能够验证所采用的提取和纯化过程不是在能够导致多糖主链中果糖去除的剧烈条件下进行的。
