Schäffler A, Büchler C, Müller-Ladner U, Herfarth H, Ehling A, Paul G, Schölmerich J, Zietz B
Department of Internal Medicine I, University of Regensburg, D-93042 Regensburg, Germany.
Horm Metab Res. 2004 Oct;36(10):702-7. doi: 10.1055/s-2004-826015.
Resistin, a peptide hormone, has been discussed controversially as a missing link between obesity and insulin resistance. In contrast to resistin mRNA expression in adipose tissue, data on human serum levels in obesity and diabetes mellitus is scarce. The physiological range of serum resistin levels, reference values or adjusted percentiles have not yet been determined, making the interpretation of serum resistin concentrations quite difficult.
Resistin serum concentrations were measured systematically by ELISA in 216 healthy controls, 555 patients with type 2 diabetes and 114 patients with type 1 diabetes. Mean values, median, and range were determined, and BMI-, gender-, and disease-adapted percentiles were calculated for all subgroups.
Age and gender did not have any influence on resistin levels. BMI and resistin levels were positively correlated in healthy controls (p = 0.02), albeit with a weak correlation coefficient. This correlation was absent in patients with type 1 and type 2 diabetes. In both genders, healthy controls had significantly higher resistin levels than patients with type 1 and type 2 diabetes (7.9 +/- 0.2 ng/ml vs. 5.7 +/- 0.2 ng/ml and 5.5 +/- 0.1 ng/ml, respectively; p < 0.0001). There was no correlation between resistin levels and occurrence of diabetic retinopathy or nephropathy.
Serum resistin levels can be measured by ELISA over a broad range from 0.6 ng/ml up to 27.7 ng/ml, suggesting that percentiles might be helpful in the interpretation of an individuals resistin value. While age and gender do not influence resistin levels, BMI and occurrence of diabetes have to be considered.
抵抗素作为一种肽类激素，一直以来都是肥胖与胰岛素抵抗之间缺失环节的争议性话题。与脂肪组织中抵抗素mRNA表达情况不同，关于肥胖和糖尿病患者血清水平的数据较少。血清抵抗素水平的生理范围、参考值或校正百分位数尚未确定，这使得血清抵抗素浓度的解读颇具难度。
采用酶联免疫吸附测定法（ELISA）系统检测了216名健康对照者、555名2型糖尿病患者和114名1型糖尿病患者的血清抵抗素浓度。确定了平均值、中位数和范围，并为所有亚组计算了根据体重指数（BMI）、性别和疾病调整后的百分位数。
年龄和性别对抵抗素水平无任何影响。在健康对照者中，BMI与抵抗素水平呈正相关（p = 0.02），尽管相关系数较弱。1型和2型糖尿病患者中不存在这种相关性。在男女两性中，健康对照者的抵抗素水平均显著高于1型和2型糖尿病患者（分别为7.9±0.2 ng/ml vs. 5.7±0.2 ng/ml和5.5±0.1 ng/ml；p < 0.0001）。抵抗素水平与糖尿病视网膜病变或肾病的发生之间无相关性。
采用ELISA法可在0.6 ng/ml至27.7 ng/ml的广泛范围内检测血清抵抗素水平，这表明百分位数可能有助于解读个体的抵抗素值。虽然年龄和性别不影响抵抗素水平，但必须考虑BMI和糖尿病的发生情况。
