Koksel Oguz, Ozdulger Ali, Ercil Menderes, Tamer Lulufer, Ercan Bahadir, Atik Ugur, Cinel Leyla, Cinel Ismail, Kanik Arzu
Department of Thoracic Surgery, University of Mersin School of Medicine, Mersin, Turkey.
Exp Lung Res. 2004 Sep;30(6):431-46. doi: 10.1080/01902140490476319.
The antioxidant and anti-inflammatory properties of N-acetylcysteine has been documented in many experimental lung injury models. Because intravenous injection of oleic acid induces histopathologic changes similar to those seen in human acute lung injury or acute respiratory distress syndrome, the authors evaluated the effects of N-acetylcysteine (NAC) on oxidative stress and lung damage in an oleic acid (OA)-induced lung injury model. Thirty-five rats were divided into 5 groups as sham, NAC, OA, pre-OA-NAC, and post-OA-NAC. Lung damage was induced by intravenous administration of oleic acid. Pre-OA-NACgroup received intravenous (IV) N-acetylcysteine 15 minutes before oleic acid infusion and post-OA-NAC group received IV N-acetylcysteine 2 hours after oleic acid infusion. In both of the N-acetylcysteine treatment groups, blood and tissue samples were collected 4 hours after oleic acid infusion, independent from the time of N-acetylcysteine infusion. In other groups, blood and tissue samples were collected 4 hours after ethanol, NAC, or OA infusions. Serum myeloperoxidase activity, total antioxidant capacity, malondialdehyde levels, and lung tissue Na+ - K+ ATPase activity were measured and light microscopic analyses of lung specimens were performed. The administration of N-acetylcysteine significantly restored Na+ - K+ ATPase activity and total antioxidant capacity levels and ameliorated lung architecture. N-acetylcysteine has been shown to have some attenuating effects in experimental animal studies. However, further investigations are necessary to suggest N-acetylcysteine as a treatment agent in critically ill patients with lung injury.
N-乙酰半胱氨酸的抗氧化和抗炎特性已在许多实验性肺损伤模型中得到证实。由于静脉注射油酸会诱发与人类急性肺损伤或急性呼吸窘迫综合征相似的组织病理学变化，因此作者在油酸（OA）诱导的肺损伤模型中评估了N-乙酰半胱氨酸（NAC）对氧化应激和肺损伤的影响。35只大鼠被分为假手术组、NAC组、OA组、OA前NAC组和OA后NAC组，共5组。通过静脉注射油酸诱导肺损伤。OA前NAC组在注入油酸前15分钟静脉注射N-乙酰半胱氨酸，OA后NAC组在注入油酸后2小时静脉注射N-乙酰半胱氨酸。在两个N-乙酰半胱氨酸治疗组中，在注入油酸4小时后采集血液和组织样本，与N-乙酰半胱氨酸注入时间无关。在其他组中，在注入乙醇、NAC或OA 4小时后采集血液和组织样本。测量血清髓过氧化物酶活性、总抗氧化能力、丙二醛水平和肺组织钠钾ATP酶活性，并对肺标本进行光镜分析。N-乙酰半胱氨酸的给药显著恢复了钠钾ATP酶活性和总抗氧化能力水平，并改善了肺结构。在实验动物研究中，N-乙酰半胱氨酸已显示出一些减轻作用。然而，有必要进一步研究，以推荐将N-乙酰半胱氨酸作为肺损伤重症患者的治疗药物。
