Dooher Julia E, Lingappa Jaisri R
Department of Pathobiology, University of Washington, Seattle, WA, USA.
J Med Primatol. 2004 Oct;33(5-6):272-80. doi: 10.1111/j.1600-0684.2004.00075.x.
We recently demonstrated that capsids from three main primate lentiviral lineages appear to form via a pathway of assembly intermediates in primate cells. Retroviral capsid assembly intermediates were initially identified and characterized using a cell-free system for assembly of immature HIV-1 capsids. Because cell-free capsid assembly systems are useful tools, we are interested in developing such systems for other primate lentiviruses besides HIV-1. Here we extend previous cell-free studies by showing that Gag proteins of HIV-2, from a second primate lentiviral lineage, progress from early intermediates to late intermediates and completed capsids over time. Additionally, we demonstrate that Gag proteins of SIVagm, from a third primate lentiviral lineage, associate with the cellular factor HP68 and complete assembly in this system. Therefore, cell-free systems reproduce assembly of Gag from three main primate lentiviral lineages, and can be used to compare mechanistic features of capsid assembly of genetically divergent primate lentiviruses.
我们最近证明，来自三个主要灵长类慢病毒谱系的衣壳似乎是通过灵长类细胞中的组装中间体途径形成的。逆转录病毒衣壳组装中间体最初是使用用于组装未成熟HIV-1衣壳的无细胞系统鉴定和表征的。由于无细胞衣壳组装系统是有用的工具，我们有兴趣为除HIV-1之外的其他灵长类慢病毒开发此类系统。在这里，我们通过表明来自第二个灵长类慢病毒谱系的HIV-2的Gag蛋白随着时间的推移从早期中间体发展到晚期中间体并形成完整的衣壳，扩展了先前的无细胞研究。此外，我们证明来自第三个灵长类慢病毒谱系的SIVagm的Gag蛋白与细胞因子HP68相关联并在该系统中完成组装。因此，无细胞系统重现了来自三个主要灵长类慢病毒谱系的Gag组装，可用于比较遗传上不同的灵长类慢病毒衣壳组装的机制特征。
