Rvb1p/Rvb2p招募Arp5p并组装一个功能性的Ino80染色质重塑复合物。
Rvb1p/Rvb2p recruit Arp5p and assemble a functional Ino80 chromatin remodeling complex.
作者信息
Jónsson Zophonías O, Jha Sudhakar, Wohlschlegel James A, Dutta Anindya
机构信息
University of Virginia School of Medicine, Jordan 1240, 1300 Jefferson Park Avenue, Charlottesville, VA 22908, USA.
出版信息
Mol Cell. 2004 Nov 5;16(3):465-77. doi: 10.1016/j.molcel.2004.09.033.
The Rvb1p and Rvb2p (or TIP48 and TIP49) nuclear ATP binding proteins are universally conserved in eukaryotes and essential for viability of yeasts. Rvbp associate with each other as a double hexamer, with YHR034c and with two complexes involved in chromatin remodeling, Ino80.com and Swr1.com. Loss of Rvb1p or Ino80p affects many yeast promoters similarly. Rvbp are not essential for the recruitment of Ino80p to promoters but are essential for the catalytic activity of Ino80.com. Loss of Rvbp leads to loss of the functionally critical Arp5p in Ino80.com. Rvb2p associates with Arp5p in vitro in a reaction dependent on the presence of ATP and Ino80p. Therefore, Rvbp are required for the structural and functional integrity of the Ino80 chromatin remodeling complex.
Rvb1p和Rvb2p（即TIP48和TIP49）核ATP结合蛋白在真核生物中普遍保守，对酵母的生存能力至关重要。Rvbp以双六聚体的形式相互结合，与YHR034c以及与参与染色质重塑的两个复合物Ino80.com和Swr1.com结合。Rvb1p或Ino80p的缺失对许多酵母启动子的影响相似。Rvbp对于Ino80p募集到启动子并非必需，但对于Ino80.com的催化活性至关重要。Rvbp的缺失会导致Ino80.com中功能关键的Arp5p丢失。Rvb2p在体外与Arp5p结合，该反应依赖于ATP和Ino80p的存在。因此，Rvbp是Ino80染色质重塑复合物结构和功能完整性所必需的。
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