PNA-3'DNA连接单体的新合成方法，这是用于获得PNA/DNA嵌合体的有用构建模块。

New synthesis of PNA-3'DNA linker monomers, useful building blocks to obtain PNA/DNA chimeras.

作者信息

Musumeci Domenica, Roviello Giovanni N, Valente Margherita, Sapio Roberto, Pedone Carlo, Bucci Enrico M

机构信息

Istituto di Biostrutture e Bioimmagini-CNR, via Mezzocannone 16, 80134 Napoli, Italy.

出版信息

Biopolymers. 2004;76(6):535-42. doi: 10.1002/bip.20135.

DOI:10.1002/bip.20135

PMID:15526336

Abstract

A new synthetic strategy to get the PNA-3'DNA linker with the monomethoxytrityl (Mmt) group as temporary protection of the backbone to be used for the synthesis of PNA/DNA chimeras was employed and a convenient strategy to obtain Mmt PNA monomers was developed. The synthetic strategies take advantage of the introduction of the acid-labile Mmt-protecting group in the first step.

摘要

采用了一种新的合成策略来获得带有单甲氧基三苯甲基（Mmt）基团的PNA-3'DNA连接体，该基团作为主链的临时保护基，用于合成PNA/DNA嵌合体，并开发了一种获得Mmt保护的PNA单体的简便策略。这些合成策略利用了第一步中引入的对酸不稳定的Mmt保护基团。