携带5-溴尿嘧啶和5-甲基胞嘧啶的DNA-PNA嵌合体的合成及杂交特性

Synthesis and hybridization properties of DNA-PNA chimeras carrying 5-bromouracil and 5-methylcytosine.

作者信息

Ferrer E, Shevchenko A, Eritja R

机构信息

European Molecular Biology Laboratory, Heidelberg, Germany.

出版信息

Bioorg Med Chem. 2000 Feb;8(2):291-7. doi: 10.1016/s0968-0896(99)00308-9.

DOI:10.1016/s0968-0896(99)00308-9

PMID:10722151

Abstract

The preparation of 5-bromouracil and 5-methylcytosine peptide nucleic acid (PNA) monomers is described. These PNA monomers were used for the preparation of several DNA-PNA chimeras and their hybridization properties are described. The substitution of cytosine by 5-methylcytosine in DNA-PNA chimeras increased duplex stability while substitution of thymine by 5-bromouracil maintained it. Moreover, binding of DNA-PNA chimeras to double-stranded DNA to form triple helices was studied. In contrast to DNA, the presence of 5-methylcytosine and 5-bromouracil in DNA-PNA chimeras destabilized triple helix.

摘要

描述了5-溴尿嘧啶和5-甲基胞嘧啶肽核酸（PNA）单体的制备方法。这些PNA单体用于制备几种DNA-PNA嵌合体，并描述了它们的杂交特性。在DNA-PNA嵌合体中用5-甲基胞嘧啶取代胞嘧啶可提高双链稳定性，而用5-溴尿嘧啶取代胸腺嘧啶则可维持双链稳定性。此外，还研究了DNA-PNA嵌合体与双链DNA结合形成三链螺旋的情况。与DNA不同，DNA-PNA嵌合体中5-甲基胞嘧啶和5-溴尿嘧啶的存在会使三链螺旋不稳定。