Lu ZhaoHui, Hu LiPing, Evers Stefan, Chen Jie, Shen Yan
Department of Pathology, Peking Union Medical College Hospital, Beijing, PR China.
Proteomics. 2004 Dec;4(12):3975-88. doi: 10.1002/pmic.200300863.
Due to poor prognosis and lack of effective treatment, pancreatic carcinoma (PC) is a devastating disease. With the goal of contributing to an improved detection, prevention and treatment of the disease, a comparative proteome analysis of PC and normal tissue was carried out. Paired tissue extracts from 12 patients (pancreatic adenocarcinoma and adjacent healthy tissue) were separated by two-dimensional electrophoresis. Differential protein expression was analyzed by gel comparison with the help of image analysis software. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Seventy proteins were more strongly expressed (mostly two-fold or more) in cancerous tissue, while 41 were stronger in normal pancreas respectively. Those spots highly expressed in PC were confirmed in gels from independent individual samples. Among them were several cytoskeletal proteins, small GTP-binding proteins, and members of the S100 protein family etc. Nine proteins had been reported in previous nuclear acid-based studies. The levels of two proteins were confirmed by immunohistochemistry. One of them, fascin, was detected in 13 out of 21 carcinoma and negative in all normal pancreas samples. Moreover, fascin expression was related to the differentiation of pancreatic carcinoma.
由于预后较差且缺乏有效的治疗方法，胰腺癌（PC）是一种极具毁灭性的疾病。为了有助于改善该疾病的检测、预防和治疗，我们对胰腺癌组织和正常组织进行了比较蛋白质组分析。从12名患者（胰腺腺癌和相邻健康组织）获取的配对组织提取物通过二维电泳进行分离。借助图像分析软件，通过凝胶比较分析差异蛋白表达。通过基质辅助激光解吸/电离飞行时间质谱法鉴定差异表达的斑点。70种蛋白质在癌组织中表达更强（大多为两倍或更高），而41种在正常胰腺中表达更强。在独立个体样本的凝胶中证实了那些在胰腺癌中高表达的斑点。其中包括几种细胞骨架蛋白、小GTP结合蛋白和S100蛋白家族成员等。在先前基于核酸的研究中已报道了9种蛋白质。通过免疫组织化学证实了两种蛋白质的水平。其中一种，即丝聚蛋白，在21例癌组织中的13例中检测到，在所有正常胰腺样本中均为阴性。此外，丝聚蛋白的表达与胰腺癌的分化有关。
