Tian Yishen, Hao Lijie, Wang Chao, Yang Xiaoyan, Liu Shufeng
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Nanomaterials (Basel). 2019 Jan 18;9(1):111. doi: 10.3390/nano9010111.
Development of simple, convenient, and sensitive assay methods for pyrophosphatase (PPase) activity is of importance, for disease diagnosis and drug discovery. Herein, a simple, rapid, label-free, and sensitive fluorescence sensor for PPase activity assay is developed, using Cu doping-induced quantum dot (QD) photoluminescence as a signal reporter. The Cu doping of ZnSe QD can induce a dopant-dependent emission response, which will be inhibited after the premixing of Cu with pyrophosphate (PPi), to form a Cu-PPi complex. Then, the hydrolysis of PPi into phosphate (Pi), specifically catalyzed by PPase, liberates the free Cu to regain the QD doping for the fluorescence response, which is highly dependent on the PPase activity. The PPase can be sensitively and selectively assayed, with a detection limit of 0.1 mU/mL. The developed sensing strategy can be also employed for the PPase inhibitor screening. Thus, the current QD doping-based sensing strategy offers an efficient and promising avenue for Cu, PPi, or PPase-related target analysis, and might hold great potential for the further applications in the clinical disease diagnosis.
开发用于焦磷酸酶（PPase）活性的简单、便捷且灵敏的检测方法对于疾病诊断和药物发现具有重要意义。在此，利用铜掺杂诱导量子点（QD）光致发光作为信号报告器，开发了一种用于PPase活性检测的简单、快速、无标记且灵敏的荧光传感器。ZnSe量子点的铜掺杂可诱导依赖于掺杂剂的发射响应，在铜与焦磷酸（PPi）预混合形成铜 - PPi络合物后，该响应将受到抑制。然后，由PPase特异性催化的PPi水解为磷酸盐（Pi），释放出游离的铜，从而恢复量子点掺杂以产生荧光响应，该响应高度依赖于PPase活性。PPase能够被灵敏且选择性地检测，检测限为0.1 mU/mL。所开发的传感策略还可用于PPase抑制剂的筛选。因此，当前基于量子点掺杂的传感策略为铜、PPi或PPase相关的靶标分析提供了一条高效且有前景的途径，并且在临床疾病诊断的进一步应用中可能具有巨大潜力。
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