Aleksandrova M A, Poltavtseva R A, Revishchin A V, Korochkin L I, Sukhikh G T
Laboratory of Experimental Neurobiology, Institute of Developmental Biology, Russian Academy of Sciences, Russia.
Neurosci Behav Physiol. 2004 Sep;34(7):659-62. doi: 10.1023/b:neab.0000036003.22350.3b.
The aim of the present work was to study human neural stem/progenitor cells (SPC) cultured in vitro and their potential to survive, migrate, and differentiate after transplantation into adult rat brain. SPC were extracted from the brains of nine-week human embryos and were cultured in selective medium for three weeks. Transplantation was with suspensions of cells or whole neurospheres; these were studied four weeks after transplantation into the hippocampus, striatum, and lateral ventricles of adult rats. Analysis of transplanted cells was based on various histological and immunohistological staining methods: bisbenzimide, bromodeoxyuridine, and antibodies to human nuclei, vimentin, beta-tubulin, neurofilaments, and glial fibrillar acidic protein, which allowed us to make independent assessments of their state and differentiation. Transplanted SPC from human brains survived well for one month in all areas of adult rat brain without immunosuppression. Cells from suspension transplants migrated intensely and differentiated into neurons and gliocytes. At the same time, transplants of whole neurospheres showed limited or no migration because of the development of a glial barrier.
本研究的目的是体外培养人神经干细胞/祖细胞（SPC），并研究其移植到成年大鼠脑内后存活、迁移及分化的潜能。从9周龄人类胚胎脑中提取SPC，并在选择性培养基中培养3周。移植采用细胞悬液或完整神经球；将其移植到成年大鼠的海马体、纹状体和侧脑室4周后进行研究。基于多种组织学和免疫组织化学染色方法对移植细胞进行分析：双苯甲酰胺、溴脱氧尿苷以及针对人细胞核、波形蛋白、β微管蛋白、神经丝和胶质纤维酸性蛋白的抗体，这些使我们能够独立评估它们的状态和分化情况。来自人脑的移植SPC在成年大鼠脑的所有区域无需免疫抑制即可良好存活1个月。悬浮移植的细胞强烈迁移并分化为神经元和神经胶质细胞。与此同时，由于胶质屏障的形成，完整神经球的移植显示出有限的迁移或无迁移。
