Zhu Guowei, Sun Chongran, Liu Weiguo
Department of Neurosurgery, Second Affiliated Hospital of Zhejiang University Medical College, Hangzhou 310009, Zhejiang Province, China.
Neural Regen Res. 2012 Jul 5;7(19):1483-7. doi: 10.3969/j.issn.1673-5374.2012.19.006.
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for β-III tubulin (neuronal marker), and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for O4 (oligodendrocytic marker). At 14 days, there were 5.6% nestin-, 9.6% β-III tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibrillary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.
在本研究中，收集孕16天胎鼠大脑皮质的细胞，并用20μg/L神经营养因子-3进行培养。培养7天后，免疫细胞化学染色显示，22.4%的细胞巢蛋白呈阳性，10.5%的细胞β-III微管蛋白（神经元标志物）呈阳性，60.6%的细胞胶质纤维酸性蛋白呈阳性，但没有细胞O4（少突胶质细胞标志物）呈阳性。在第14天，有5.6%的细胞巢蛋白呈阳性、9.6%的细胞β-III微管蛋白呈阳性、81.1%的细胞胶质纤维酸性蛋白呈阳性以及2.2%的细胞O4呈阳性。在未用神经营养因子-3处理的细胞中，一些细胞巢蛋白呈阳性，而大多数细胞胶质纤维酸性蛋白呈阳性染色。我们的实验结果表明，神经营养因子-3是诱导神经干细胞分化为神经元和少突胶质细胞的关键因素。
