Miroshnikov A I
Biofizika. 2004 Sep-Oct;49(5):866-71.
To study the causes of the biological effect of electrochemically activated solutions, nutrient growth media M 9 were prepared using catholyte and anolyte solutions containing separate components of the nutrient medium, such as distilled water, phosphate buffer, phosphate buffer with chlorides (NaCl, NH4Cl), and chlorides. The biological activity of different nutrient media was assessed by a comparison with the stimulation or inhibition of the growth of Escherichia coli cells in the catholyte and anolyte of the complete nutrient medium M 9. It was shown that medium M 9 prepared on the catholytes of different initial solutions acquired the stimulating properties only if the initial solution contained salts containing chlorine. The stimulating effect of the initial solution was 18-24%. Electrochemical treatment of solutions containing no chlorides (distilled water, phosphate buffer) and subsequent addition of the components of nutrient medium to exposed solutions had neither a stimulating nor the inhibiting effect on cell growth. The cultivation of cells in a nutrient medium based on the catholyte of preliminarily treated hydrochloric acid showed that it is the presence of chlorine ions in solution during electrolysis that causes the stimulating effect of the nutrient medium based on the catholyte. The formation of oxidizers and the inhibitory effect of the anolyte described previously was also observed if the solution contained chlorine ions during electrolysis. Possible mechanisms of the biological effect of catholytes containing chlorides during electrolysis were discussed.
为了研究电化学活化溶液生物效应的原因，使用含有营养培养基单独成分的阴极电解液和阳极电解液制备了营养生长培养基M9，这些单独成分包括蒸馏水、磷酸盐缓冲液、含氯化物（氯化钠、氯化铵）的磷酸盐缓冲液以及氯化物。通过比较完全营养培养基M9的阴极电解液和阳极电解液中大肠杆菌细胞生长的刺激或抑制情况，评估了不同营养培养基的生物活性。结果表明，只有当初始溶液含有含氯盐时，用不同初始溶液的阴极电解液制备的培养基M9才具有刺激特性。初始溶液的刺激效果为18% - 24%。对不含氯化物的溶液（蒸馏水、磷酸盐缓冲液）进行电化学处理，然后向处理后的溶液中添加营养培养基成分，对细胞生长既无刺激作用也无抑制作用。在基于预处理盐酸阴极电解液的营养培养基中培养细胞表明，电解过程中溶液中氯离子的存在导致了基于阴极电解液的营养培养基产生刺激作用。如果电解过程中溶液含有氯离子，还会观察到之前所述的氧化剂的形成和阳极电解液的抑制作用。讨论了电解过程中含氯化物阴极电解液生物效应的可能机制。
